Probing the Biophysical Properties of Primary Breast Tumor-Derived Fibroblasts

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• 作者：Turi A. Alcoser (1)
  Francois Bordeleau (1)
  Shawn P. Carey (1)
  Marsha C. Lampi (1)
  Daniel R. Kowal (2)
  Sahana Somasegar (1)
  Sonal Varma (3)
  Sandra J. Shin (3)
  Cynthia A. Reinhart-King (1)
  
  1. Department of Biomedical Engineering ; Cornell University ; 302 Weill Hall ; 526 Campus Rd ; Ithaca ; NY ; 14853 ; USA
  2. Department of Statistical Science ; Cornell University ; Ithaca ; NY ; 14853 ; USA
  3. Department of Pathology and Laboratory Medicine ; The New York Presbyterian Hospital-Weill Cornell Medical College ; New York ; NY ; 10065 ; USA
  
• 关键词：Stromal cells ; Migration ; Collagen ; Traction force microscopy ; Spheroid ; Confocal reflectance
• 刊名：Cellular and Molecular Bioengineering
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：8
• 期：1
• 页码：76-85
• 全文大小：4,685 KB
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• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering
  Mechanics
  Continuum Mechanics and Mechanics of Materials
  Biophysics and Biomedical Physics
  Cell Biology
  
• 出版者：Springer New York
• ISSN：1865-5033

文摘

As cancer progresses, cells must adapt to a new and stiffer environment, which can ultimately alter how normal cells within the tumor behave. In turn, these cells are known to further aid tumor progression. Therefore, there is potentially a unique avenue to better understand metastatic potential through single-cell biophysical assays performed on patient-derived cells. Here, we perform biophysical characterization of primary human fibroblastic cells obtained from mammary carcinoma and normal contralateral tissue. Through a series of tissue dissociation, differential centrifugation and trypsinization steps, we isolate an adherent fibroblastic population viable for biomechanical testing. 2D TFM and 3D migration measurements in a collagen matrix show that fibroblasts obtained from patient tumors generate more traction forces and display improved migration potential than their counterparts from normal tissue. Moreover, through the use of an embedded spheroid model, we confirmed the extracellular matrix remodeling behavior of primary cells isolated from carcinoma. Overall, correlating biophysical characterization of normal- and carcinoma-derived samples from individual patients along with patient outcome may become a powerful approach to further our comprehension of metastasis and ultimately design drug targets on a patient-specific basis.