The spatial variability of the vertical structure of bioluminescence intensity has been studied along the Crimean coast in the spring period. The location of a layer with a high intensity of bioluminescence has been determined and its dependence on hydrological characteristics and thermohaline structure of waters is shown. Structurization of the spatial variability of bioluminescence intensity has been made. Four regions that are distinguished by the pattern of the vertical distribution of the bioluminescence intensity have been distinguished in coastal waters of the Crimean Peninsula.KeywordsbioluminescenceBlack Seavertical structurehydrological characteristics