Using eDNA to experimentally test ungulate browsing preferences

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• 作者：Ruth V. Nichols ; Joris P. G. M. Cromsigt ; G枚ran Spong
• 关键词：Herbivory ; DNA ; Deer ; Sweden
• 刊名：SpringerPlus
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：4
• 期：1
• 全文大小：1144KB
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• 作者单位：Ruth V. Nichols (1) (2)
  Joris P. G. M. Cromsigt (1) (3)
  G枚ran Spong (1) (4)
  
  1. Molecular Ecology Research Group, Department of Wildlife, Fish, and Environmental Studies, Swedish University of Agricultural Sciences, Skogsmarksgr盲nd, 90183, Ume氓, Sweden
  2. Department of Ecology and Evolutionary Biology, University of California Santa Cruz (UCSC), Santa Cruz, CA, 95064, USA
  3. Department of Zoology, Centre for African Conservation Ecology, Nelson Mandela Metropolitan University, NMMU, PO Box 77000, Port Elizabeth, 6031, South Africa
  4. Fisheries, Wildlife, and Conservation Biology, College of Natural Resources, North Carolina State University, Raleigh, NC, 27695-7646, USA
  
• 刊物类别：Science, general;
• 刊物主题：Science, general;
• 出版者：Springer International Publishing
• ISSN：2193-1801

文摘

Large herbivores may affect ecosystem processes and states, but such effects can be difficult to quantify, especially within multispecies assemblages. To better understand such processes and improve our predictive ability of systems undergoing change, herbivore diets can be studied using controlled feeding trials (or cafeteria tests). With some wildlife, such as large herbivores, it is impractical to empirically verify these findings, because it requires visually observing animals in forested environments, which can disturb them from their natural behaviors. Yet, in field-based cafeteria trials it is nearly impossible to differentiate selection between herbivore species that forage on similar plants and make very similar bite marks. However, during browsing ungulates leave saliva residue which includes some buccal cells and DNA that can be extracted for species identification. Here we used a newly developed eDNA-based method (biteDNA) to test the browsing preferences of four sympatric ungulate species in the wild. Overall, food preferences varied between species, but all species strongly preferred deciduous over coniferous species. Our method allows the study of plant-animal interactions in multispecies assemblages at very fine detail. Keywords Herbivory DNA Deer Sweden