Intra-specific relationships among Tibetan Eared-pheasants based on randomly amplified polymorphic DNA (RAPD) analysis

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• 作者：Wei Ding (1)
  Zhengwang Zhang (1)
  Jiang Chang (1)
  Er Zhang (2)
  Xiushan Wu (2)
  Jinguo Zhang (2)
  
• 关键词：Tibetan Eared ; pheasant ; Crossoptilon harmani ; genetic relationship ; random amplified polymorphic DNA (RAPD)
• 刊名：Frontiers in Biology
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：1
• 期：4
• 页码：394-397
• 全文大小：325KB
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• 作者单位：Wei Ding (1)
  Zhengwang Zhang (1)
  Jiang Chang (1)
  Er Zhang (2)
  Xiushan Wu (2)
  Jinguo Zhang (2)
  
  1. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Beijing Normal University, Beijing, 100875, China
  2. Beijing Zoo, Beijing, 100044, China
  
• ISSN：1674-7992

文摘

The Tibetan Eared-pheasant Crossoptilon harmani is a rare species native to China. A captive population has been established in the Beijing Zoo since 1999. In order to determine the kinship of the offsprings in 2001, randomly amplified polymorphic DNA (RAPD) was used to examine the parenthood of seven Tibetan Eared-pheasants in the Beijing Zoo. To amplify the genomic DNA of each individual, 53 arbitrary primers were selected. The results of amplifications showed that 14 primers had clear and distinct RAPD patterns. Totally, 226 amplified fragments were generated by RAPD in this study. Cluster analysis of the seven Tibetan Eared-pheasants indicated that all the four young birds had the same father (No.5 male). This study provides a practical method to determine the relationship of offsprings whose parents are unknown in birds.