A novel bi-directional promoter cloned from melon and its activity in cucumber and tobacco

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• 作者：Cuiyan Wang (1)
  Dongfeng Ding (1)
  Ruixiang Yan (1) (2)
  Xiaoju Yu (1)
  Weidong Li (3)
  Minggang Li (1)
  
• 关键词：bi ; directional promoter ; double promoter ; transient transformation ; uidA reporter gene
• 刊名：Journal of Plant Biology
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：51
• 期：2
• 页码：108-115
• 全文大小：1255KB
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• 作者单位：Cuiyan Wang (1)
  Dongfeng Ding (1)
  Ruixiang Yan (1) (2)
  Xiaoju Yu (1)
  Weidong Li (3)
  Minggang Li (1)
  
  1. Key Laboratory for Bioactive Material of Ministry of Education, Institute of Molecular Biology Nankai University, 300071, Tianjin, China
  2. National Engineering and Technology Research Center for Agricultural Products Freshness Protection, 300384, Tianjin, China
  3. The University of Tokyo, Tokyo, Japan
  

文摘

A bi-directional promoter, DP, was cloned by PCR amplification using the genomic DNA of melon as template. Analysis of itscis-acting elements in both directions revealed a series of inducible regulatory elements and some enhancer elements. To evaluate its transcriptional activity, DP in both directions was then cloned into vector pBI121 to replace the CaMV 35S promoter. DP in both directions also was inserted downstream of CaMV 35S to investigate whether the double promoter might affect expression of theuidA reporter gene at higher levels. Transient expression in cucumber leaves, stems, and fruits as well as in tobacco leaves and stems showed that DP in both directions drove transcription to much higher levels than did the single promoter CaMV 35S. However, activity of the double promoter was lower than the corresponding activity of the single promoter DP in both directions. These results demonstrate that DP is a natural bi-directional promoter, with much more activity than is found with the CaMV 35S promoter. Furthermore, in cucumber and tobacco, it is not suitable to insert DP in either direction downstream of the CaMV 35S promoter to form a double promoter.