Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4
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- 作者：Tomas Humhal ; Petr Kastanek ; Zuzana Jezkova…
- 关键词：Saline waste water ; Schizochytrium limacinum ; Japonochytrium marinum ; Docosahexaenoic acid ; Shake flask ; Bioreactor
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2017
- 出版时间：March 2017
- 年：2017
- 卷：40
- 期：3
- 页码：395-402
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Industrial and Production Engineering; Environmental Engineering/Biotechnology; Industrial Chemistry/Chemical Engineering; Food Science;
- 出版者：Springer Berlin Heidelberg
- ISSN：1615-7605
- 卷排序：40
文摘
Saline waste water from demineralization of cheese whey was used as the main component of waste saline medium (WSM) for cultivation of thraustochytrids. The suitability of WSM for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4 was evaluated by comparison with cultivation on nutrient medium (NM) in shake flask and fermenter cultures. Biomass productivities achieved in WSM for the thraustochytrids were comparable with those in NM for both shake flask and fermenter cultures. The maximum total lipid content (56.71% dry cell weight) and docosahexaenoic acid productivity (0.86 g/L/day) were achieved by J. marinum AN-4 grown on WSM in shake flask and fermenter cultures, respectively. A cost estimate of WSM suggests that this medium could result in lower production costs for thraustochytrid biomass and lipids and contribute to the effective reduction in saline diary process waste water.