Analysis of cuticular wax constituents and genes that contribute to the formation of ‘glossy Newhall- a spontaneous bud mutant from the wild-type ‘Newhall-navel orange

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• 作者：Dechun Liu ; Li Yang ; Qiong Zheng ; Yuechen Wang ; Minli Wang…
• 关键词：Epicuticular waxes ; Glossy mutant ; Intracuticular waxes ; Navel orange ; Wax composition ; Wax genes
• 刊名：Plant Molecular Biology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：88
• 期：6
• 页码：573-590
• 全文大小：1,634 KB
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• 作者单位：Dechun Liu (1)
  Li Yang (1)
  Qiong Zheng (1)
  Yuechen Wang (1)
  Minli Wang (1)
  Xia Zhuang (1)
  Qi Wu (1)
  Chuanfu Liu (2)
  Shanbei Liu (1)
  Yong Liu (1)
  
  1. Department of Pomology, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China
  2. Bureau of Fruit and Tea, Xinfeng County, 341600, Ganzhou, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

Navel orange (Citrus sinensis [L.] Osbeck) fruit surfaces contain substantial quantities of cuticular waxes, which have important eco-physiological roles, such as water retention and pathogen defense. The wax constituents of ripe navel orange have been studied in various reports, while the wax changes occurring during fruit development and the molecular mechanism underlying their biosynthesis/export have not been investigated. Recently, we reported a spontaneous bud mutant from the wild-type (WT) ‘Newhall-Navel orange. This mutant displayed unusual glossy fruit peels and was named ‘glossy Newhall-(MT). In this study, we compared the developmental profiles of the epicuticular and intracuticular waxes on the WT and MT fruit surfaces. The formation of epicuticular wax crystals on the navel orange surface was shown to be dependent on the accumulation of high amounts of aliphatic wax components with trace amounts of terpenoids. In sharp contrast, the underlying intracuticular wax layers have relatively low concentrations of aliphatic wax components but high concentrations of cyclic wax compounds, especially terpenoids at the late fruit developmental stages. Our work also showed that many genes that are involved in wax biosynthesis and export pathways were down-regulated in MT fruit peels, leading to a decrease in aliphatic wax component amounts and the loss of epicuticular wax crystals, ultimately causing the glossy phenotype of MT fruits.