Light affects in vitro organogenesis of Linum usitatissimum L. and its cyanogenic potential

详细信息    查看全文

• 作者：Irena Siegień (1)
  Aneta Adamczuk (1)
  Katarzyna Wróblewska (1)
  
• 关键词：Cyanogenesis ; Exogenous hormones ; Flax ; Linamarin ; Regeneration
• 刊名：Acta Physiologiae Plantarum
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：35
• 期：3
• 页码：781-789
• 全文大小：228KB
• 参考文献：1. Abbadi A, Domergue F, Bauger J, Napier JA, Welti R, Zahringer U, Cirpus P, Heinz E (2004) Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constrains on their accumulation. Plant Cell 16:2734-748 CrossRef
  2. Alam EA (2010) Phytochemical screening on calli of / Fagonia indica and / Fagonia bruguieti Dc. N Y Sci J 3:158-64
  3. Bertazza G, Baraldi R, Predieri S (1995) Light effects on in vitro rooting of pear cultivars of different rhizogenic ability. Plant Cell Tissue Organ Cult 41:139-43 CrossRef
  4. Bertram L, Lercari B (2000) Phytochrome A and phytochrome B1 control the acquisition of competence for shoot regeneration in tomato hypocotyls. Plant Cell Rep 19:604-09 CrossRef
  5. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-54 CrossRef
  6. Burns AE, Gleadow RM, Woodrow IE (2002) Light alters the allocation of nitrogen to cyanogenic glycosides in / Eucalyptus cladocalyx. Oecologia 133:288-94 CrossRef
  7. Caillot S, Rosiau E, Laplace C, Thomasset B (2009) Influence of light intensity and selection on regeneration time of transgenic flax plants. Plant Cell Rep 28:359-71 CrossRef
  8. Collinge DB, Hughes MA (1982) Developmental and physiological studies on the cyanogenic glucosides of white clover, / Trifolium repens L. J Exp Bot 33:154-61 CrossRef
  9. Cutler AJ, Sternberg M, Conn EE (1985) Properties of microsomal enzyme system from / Linum usitatissimum (linen flax) which oxidizes valine to acetone cyanohydrin and isoleucine to 2-methylbutanone cyanohydrin. Arch Biochem Biophys 238:272-79 CrossRef
  10. Dedi?ová B, Hricová A, ?amaj J, Obert B, Bobák M, Pret’ová A (2000) Shoot and embryo like structures regenerated from cultured flax ( / Linum usitatissimum L.) hypocotyl segments. J Plant Physiol 157:327-34 CrossRef
  11. Elias M, Sudhakaran PR, Nambisan B (1997) Purification and characterisation of β-cyanoalanine synthase from cassava tissues. Phytochemistry 46:469-72 CrossRef
  12. Gaisser S, Heide L (1996) Inhibition and regulation of shikonin biosynthesis in suspension cultures of / Lithospermum. Phytochemistry 41:1065-072 CrossRef
  13. Garcia R, Cidade D, Castellar A, Lips A, Magioli A, Callado C, Mansur E (2007) In vitro morphogenesis patterns from shoot apices of sugar cane are determined by light and type of growth regulator. Plant Cell Tissue Organ Cult 90:181-90 CrossRef
  14. Gleadow RM, Woodrow IE (2002) Constrains on effectiveness of cyanogenic glycosides in herbivore defense. J Chem Ecol 28:1301-313 CrossRef
  15. Gniazdowska A, Krasuska U, Bogatek R (2010) Dormancy removal in apple embryos by nitric oxide or cyanide involves modifications in ethylene biosynthetic pathway. Planta 232:1397-407 CrossRef
  16. Gomes da Cunha AC, Ferreira A (1996) Somatic embryogenesis, organogenesis and callus growth kinetics of flax. Plant Cell Tissue Organ Cult 47:1- CrossRef
  17. Hahlbrock K, Conn EE (1971) Evidence for the formation of linamarin and lotaustralin in flax seedlings by the same glucosyltransferase. Phytochemistry 10:1019-023 CrossRef
  18. Hassanein A, Dorion N (2005) Efficient plant regeneration system from leaf discs of zonal ( / Pelargonium x / hortorum) and two scented ( / P. capitatum and / P. graveolens) geraniums. Plant Cell Tissue Organ Cult 83:231-40 CrossRef
  19. H?sel W, Berlin J, Hanzlik TN, Conn EE (1985) In vitro biosynthesis of 1-(4-hydroxyphenyl)-2-nitroethane and production of cyanogenic compounds in osmotically stressed cell suspension cultures of / Escholtzia californica Cham. Planta 166:176-81 CrossRef
  20. Istock U, Lieberei R, Harms H (1990) Pattern of enzymes involved in cyanogenesis and HCN metabolism in cell cultures of / Phaseolus lunatus L. varieties. Plant Cell Tissue Organ Cult 22:105-12 CrossRef
  21. J?ger AK, McAlister BG, van Staden J (1995) Cyanogenic glycosides in leaves and callus cultures of / Schlechterina mitostemmatoides. S Afr Tydskr Plant 61:274-75
  22. Joseph T, Yeoh H-H, Loh C-S (1999) Cyanogenesis in somatic embryos and plantlets of cassava ( / Manihot esculenta Crantz). J Sci Food Agric 79:1071-074 CrossRef
  23. Kadkade P, Seibert M (1977) Phytochrome-regulated organogenesis in lettuce tissue culture. Nature 270:49-0 CrossRef
  24. Kaira C, Babbar SB (2010) Nitric oxide promotes in vitro organogenesis in / Linum usitatissimum L. Plant Cell Tissue Organ Cult 103:353-59 CrossRef
  25. Kakes P (1990) Properties and functions of the cyanogenic system in higher plants. Euphytica 48:25-3
  26. Kakes P (1991) A rapid and sensitive method to detect cyanogenesis using microtiterplates. Biochem Syst Ecol 19:519-22 CrossRef
  27. Kojima M, Poulton JE, Thayer SS, Conn EE (1979) Tissue distributions of dhurrin and of enzymes involved in its metabolism in leaves of / Sorghum bicolor. Plant Physiol 63:1022-028 CrossRef
  28. Lieberei R, Nahrstedt A, Selmar D, Gasparotto L (1986) The occurrence of lotaustralin in the genus / Hevea and changes of HCN-potential in developing organs of / Hevea brasiliensis. Phytochemistry 25:1573-578 CrossRef
  29. Liu JR, Sink KC, Dennis FG (1983) Plant regeneration from apple seedling explants and callus cultures. Plant Cell Tissue Organ Cult 2:293-04 CrossRef
  30. Liu C, Guo C, Wang Y, Ouyang F (2002) Effect of light irradiation on hairy root growth and artemisinin biosynthesis of / Artemisia annua L. Process Biochem 38:581-85 CrossRef
  31. Millam S, Obert B, Pret’ová A (2005) Plant cell and biotechnology studies in / Linum usitatissimum—a review. Plant Cell Tissue Organ Cult 82:93-03 CrossRef
  32. M?ller BL (2010) Functional diversifications of cyanogenic glucosides. Curr Opin Plant Biol 13:338-47 CrossRef
  33. Mundhara R, Rashid A (2001) Regeneration of shoot-buds on hypocotyl of / Linum seedlings: a stress-related response. Plant Sci 161:19-5 CrossRef
  34. Mundhara R, Rashid A (2002) Stimulation of shoot-bud regeneration on hypocotyl of / Linum seedlings, on a transient withdrawal of calcium: effect of calcium, cytokinin and thidiazuron. Plant Sci 162:211-14 CrossRef
  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-97 CrossRef
  36. Nahrstedt A, Kant J-D, H?sel W (1984) Aspect on the biosynthesis of the cyanogenic glucoside triglochinin in / Triglochin maritima. Planta Med 36:394-97 CrossRef
  37. Nied?wied?-Siegień I (1998) Cyanogenic glucosides in / Linum usitatissimum L. Phytochemistry 49:59-3 CrossRef
  38. Nied?wied?-Siegień I, Giersimiuk A (2001) Environmental factors affecting the cyanogenic potential of flax seedlings. Acta Physiol Plant 23:383-90 CrossRef
  39. Obert B, Benson EE, Millam S, Pret’ová A, Bremner DH (2005) Moderation of morphogenetic and oxidative stress responses in flax in vitro cultures by hydroxynonenal and desferrioxamine. J Plant Physiol 162:537-47 CrossRef
  40. Oracz K, El-Maarouf-Bouteau H, Kranner I, Bogatek R, Corbineau F, Bailly C (2009) The mechanisms involved in seed dormancy alleviation by hydrogen cyanide unravel the role of reactive oxygen species as key factors of cellular signaling during germination. Plant Physiol 150:494-05 CrossRef
  41. Pádua VLM, Fernandes LD, Oliveira DE, Mansur E (1998) Effects of auxin and light treatments of donor plants on shoot production from / Indica-type rice ( / Oryza sativa L.). In vitro Cell Dev Biol Plant 34:285-88 CrossRef
  42. Rutkowska-Krause I, Mankowska G, ?ukaszeiwcz M, Szopa J (2003) Regeneration of flax ( / Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to / Fusarium oxysporum. Plant Cell Rep 22:110-16 CrossRef
  43. Sanchez-Perez R, Jorgensen K, Motawia MS, Dicenta F, M?ller BL (2009) Tissue and cellular localization of individual beta-glycosidases using a substrate specific sugar reducing assay. Plant J 60:894-06 CrossRef
  44. Selmar D, Lieberei R, Biehl B (1988) Mobilisation and utilisation of cyanogenic glycosides. Plant Physiol 86:711-16 CrossRef
  45. Siegień I (2009) Cyanogenic compounds. In: Narwal SS, Bogatek R, Zagdańska BM, Sampietro DA, Vattuone MA (eds) Plant biochemistry. Studium Press LLC, Houston, pp 337-66
  46. Siegień I, Bogatek R (2006) Cyanide action in plants—from toxic to regulatory. Acta Physiol Plant 28:483-97 CrossRef
  47. Szechyńska-Hebda M, Skrzypek E, Dabrowska G, Biesaga-Ko?cielak J, Filek M, W?dzony M (2007) The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiol Plant 29:327-37 CrossRef
  48. Tyburski J, Tretyn A (2004) The role of light and polar auxin transport in root regeneration from hypocotyls of tomato seedling cuttings. Plant Growth Regul 42:39-8 CrossRef
  49. Verdus M-C, Thellier M, Ripol C (1997) Storage of environmental signals in flax. Their morphogenic effect as enabled by a transient depletion of calcium. Plant J 12:1399-410 CrossRef
  50. Vetter J (2000) Plant cyanogenic glycosides. Toxicon 38:11-6 CrossRef
  51. Vickery PJ, Wheeler JL, Mulcahy C (1987) Factors affecting the hydrogen cyanide potential of white clover ( / Trifolium repens L.). Aust J Agric Res 38:1053-059 CrossRef
  52. Wurtele ES, Nikolau BJ, Conn EE (1984) Tissue distribution of beta-cyanoalanine synthase in leaves. Plant Physiol 75:979-82 CrossRef
  53. Yeoh HH (1989) Kinetic properties of beta-glucosidase from cassava. Phytochemistry 28:721-24 CrossRef
  54. Yildiz M, ?zgen M (2004) The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax ( / Linum usitatissimum). Plant Cell Tissue Organ Cult 77:111-15 CrossRef
  55. Zagrobelny M, Bak S, Rasmussen AV, J?rgensen B, Naumann CM, M?ller BL (2004) Cyanogenic glucosides and plant-insect interactions. Phytochemistry 65:293-06 CrossRef
  56. Zhao P, Wang W, Feng F-S, Wu F, Yang Z-Q, Wang W-J (2007) High-frequency shoot regeneration through transverse thin cell layer culture in / Dendrobium Candidum Wall Ex Lindl. Plant Cell Tissue Organ Cult 90:131-39 CrossRef
  
• 作者单位：Irena Siegień (1)
  Aneta Adamczuk (1)
  Katarzyna Wróblewska (1)
  
  1. Institute of Biology, The University of Bialystok, ?wierkowa 20b, 15-950, Bialystok, Poland
  

文摘

The relationships between organogenesis of oil flax (Linum usitatissimum L., cv. ‘Szafir- in vitro, cyanogenic potential (HCN-p) of these tissues and light were investigated. Shoot multiplication obtained on Murashige and Skoog medium containing 0.05?mg?L? 2,4-dichloro-phenoxyacetic acid and 1?mg?L? 6-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness. Light-grown explants showed also higher rate of roots regeneration (in medium containing 1?mg?L? α-naphtaleneacetic acid and 0.05?mg L-1 BA) than dark-grown ones. The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness. The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots, and its amount was twice as high as in roots, and about threefold higher than in callus tissue. The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness. Thus, higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues. We suggest that free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency.