Translation elongation factor EF1α1 interacts with ZAD domains of transcription factors from Drosophila melanogaster

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• 作者：N. A. Zolotarev ; O. G. Maksimenko ; Yu. V. Shidlovskii ; P. G. Georgiev…
• 关键词：transcription factors ; EF1α1 ; insulators ; Drosophila ; GST ; pulldown ; mass spectrometry ; ZAD domain
• 刊名：Molecular Biology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：50
• 期：6
• 页码：895-899
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences, general; Biochemistry, general; Human Genetics;
• 出版者：Pleiades Publishing
• ISSN：1608-3245
• 卷排序：50

文摘

A large class of arthropod transcription factors is formed by proteins with a characteristic N-terminal Zinc-finger-Associated Domain (ZAD) which contains four cysteine residues that coordinate a zinc ion. The number of putative proteins with ZAD in the Drosophila genome exceeds 90, and the degree of sequence similarity between these domains can be as low as 23%. Efficient binding of ZADs from the proteins Grau, ZIPIC, and Zw5 to the translation elongation factor EF1α1 in nuclear and cytoplasmic extracts has been demonstrated. EF1α1 is probably involved in the regulation of the activity of ZAD-containing transcription factors.