H. pylori related proinflammatory cytokines contribute to the induction of miR-146a in human gastric epithelial cells

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• 作者：Na Li (1)
  Xiang Xu (2)
  Bin Xiao (1) binxiaotmmu@163.com
  En-Dong Zhu (1)
  Bo-sheng Li (1)
  Zhen Liu (1)
  Bin Tang (1)
  Quan-Ming Zou (1)
  Hua-Ping Liang (2) huaping_liang@yahoo.com.cn
  Xu-Hu Mao (1) xhmaotmmu@yeah.net
• 关键词：miR ; 146a &#8211 ; H. pylori &#8211 ; Proinflammatory cytokines &#8211 ; CagA
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：39
• 期：4
• 页码：4655-4661
• 全文大小：349.2 KB
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• 作者单位：1. Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing, 400038 People鈥檚 Republic of China2. State Key Laboratory of Trauma, Burns, and Combined Injury, Department 1, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042 China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

MicroRNAs have been implicated as a central regulator of the immune system. We have previously reported that Helicobacter pylori (H. pylori) was able to increase the expression of miR-146a, and miR-146a may negatively regulate H. pylori-induced inflammation, but the exact mechanism of how H. pylori contribute the induction of miR-146a is not clear. Here, we attempted to assess the role of H. pylori related proinflammatory cytokines including interleukin (IL)-8, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β, and cytotoxin-associated gene A (CagA) virulence factor on the induction of miR-146a. We found that IL-8, TNF-α, and IL-1β could contribute to the induction of miR-146a in gastric epithelial cell HGC-27 in NF-κB-dependent manner, while the induction of miR-146a upon H. pylori stimulation was independent of above proinflammatory cytokines. Furthermore, overexpression of miR-146a reduced H. pylori—induced IL-8, TNF-α, and IL-1β. However, CagA had no effect on the miR-146a induction. Taken together, our study suggest that proinflammatory cytokines IL-8, TNF-α, and IL-1β could contribute to the induction of miR-146a during H. pylori infection, while CagA is not necessarily required for miR-146a induction. miR-146a may function as novel negative regulators to modulate the inflammation.