Molecular pathology of pulmonary surfactants and cytokines in drowning compared with other asphyxiation and fatal hypothermia
详细信息 查看全文
- 作者：Takako Miyazato (1)
Takaki Ishikawa (1) takaki@med.osaka-cu.ac.jp
Tomomi Michiue (1)
Hitoshi Maeda (1) - 关键词：Molecular pathology – ; Drowning – ; Hypothermia – ; Immunohistochemistry – ; Real ; time RT ; PCR – ; Cytokines – ; Pulmonary surfactant ; associated protein
- 刊名：International Journal of Legal Medicine
- 出版年：2012
- 出版时间：July 2012
- 年：2012
- 卷：126
- 期：4
- 页码：581-587
- 全文大小：537.8 KB
- 参考文献：1. Golden FS, Tipton MJ, Scott RC (1997) Immersion, near-drowning and drowning. Br J Anaesth 79:214–225
2. Pfeiffer H, Du Chesne A, Brinkmann B (2006) An unusual case of homicidal near drowning followed by electrocution. Int J Legal Med 120:36–41
3. Zhu BL, Ishida K, Fujita MQ, Maeda H (2000) Immunohistochemical investigation of a pulmonary surfactant in fatal mechanical asphyxia. Int J Legal Med 113:268–271
4. Zhu BL, Ishida K, Quan L, Fujita MQ, Maeda H (2000) Immunohistochemistry of pulmonary suractant apoprotein A in forensic autopsy: reassessment in relation to the causes of death. Forensic Sci Int 113:193–197
5. Zhu BL, Ishida K, Oritani S, Quan L, Taniguchi M, Li DR, Fujita MQ, Maeda H (2001) Immunohistochemical investigation of pulmonary surfactant-associated protein A in fire victims. Legal Med (Tokyo) 3:23–28
6. Zhu BL, Ishida K, Quan L, Li DR, Taniguchi M et al (2002) Pulmonary immunohistochemistry and serum levels of a surfactant-associated protein A in fatal drowning. Legal Med 4:1–6
7. Perez-Carceles MD, Sibon A, Vizcaya MA, Osuna EM, Fomez-Zapata M et al (2008) Histological findings and immunohistochemical surfactant protein A (SP-A) expression in asphyxia: its application in the diagnosis of drowning. Histol Histopathol 23:1061–1068
8. Ishida K, Zhu BL, Maeda H (2000) Novel approach to quantitative reverse transcription PCR assay of mRNA component in autopsy material using the TaqMan fluorogenic detection system. Forensic Sci Int 113:127–131
9. Ishida K, Zhu BL, Maeda H (2002) A quantitative RT-PCR assay of surfactant-associated protein A1 and A2 mRNA transcripts as a diagnostic tool for acute asphyxial death. Legal Med (Tokyo) 4:7–12
10. Ishida K, Zhu BL, Maeda H (2005) TaqMan fluorogenic detection system to analyze gene transcription in autopsy material. Methods Mol Biol 291:415–421
11. Grenfell S, Smithers N, Witham S, Shaw A, Graber P et al (1991) Analysis of mutations in the putative nuclear localization sequence of interleukin-1 beta. Biochem J 280:111–116
12. Rojas M, Olivier M, Gros P, Barrera LF, Garc铆a LF (1999) TNF-alpha and IL-10 modulate the induction of apoptosis by virulent Mycobacterium tuberculosis in murine macrophages. J Immunol 62:122–131
13. Matthew CH, Danica PD, Scott HV et al (2009) Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39. J Clin Invest 119:1136–1149
14. Gubern C, Hurtado O, Rodr铆guez R et al (2009) Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia. BMC Mol Biol 10:57
15. Orlov SV, Mogilenko DA, Shavva VS, Dizhe EB, Ignatovich IA et al (2010) Effect of TNFalpha on activities of different promoters of human apolipoprotein A-I gene. Biochem Biophys Res Commun 398:224–230
16. Kim JM, Brannan CI, Copeland NG, Jenkins NA, Khan TA et al (1992) Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes. J Immunol 148:3618–3623
17. Crouch E, Persson A, Chang D, Heuser J (1994) Molecular structure of pulmonary surfactant protein D (SP-D). J Biol Chem 269:17311–17319
18. Koppelkamm A, Vennemann B, Fracasso T, Lutz-Bonengel S, Schmidt U, Heinrich M (2010) Validation of adequate endogenous reference genes for the normalization of qPCR gene expression data in human post mortem tissue. Int J Legal Med 124:371–380
19. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by genometric averaging of multiple internal control genes. Genome Biol 18:3
20. Ishikawa T, Quan L, Li DR, Zhao D, Michiue T et al (2008) Postmortem biochemistry and immunohistochemistry of adrenocorticotropic hormone with special regard to fatal hypothermia. Forensic Sci Int 179:147–151
21. Ishikawa T, Hamel M, Zhu BL, Li DR, Zhao D et al (2008) Comparative evaluation of postmortem serum concentrations of neopterin and C-reactive protein. Forensic Sci Int 179:135–143
22. Yoshida C, Ishikawa T, Michiue T, Quan L, Maeda H (2009) Postmortem biochemistry and immunohistochemistry of chromogranin A as a stress marker with special regard to fatal hypothermia and hyperthermia. Int J Legal Med 125:11–20
23. Zhao D, Zhu BL, Ishikawa T, Li DR, Michiue T et al (2006) Quantitative RT-PCR assays of hypoxia-inducible factor-1alpha, erythropoietin and vascular endothelial growth factor mRNA transcripts in the kidneys with regard to the cause of death in medicolegal autopsy. Legal Med 8:258–263
24. Zhao D, Ishikawa T, Quan L, Li DR, Michiue T et al (2009) Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases. Forensic Sci Int 177:176–183 - 作者单位：1. Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8585, Japan
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Forensic Medicine
Medical Law
Medicine/Public Health, general - 出版者：Springer Berlin / Heidelberg
- ISSN：1437-1596
文摘
Drowning involves complex fatal factors, including asphyxiation and electrolyte/osmotic disturbances, as well as hypothermia in cold water. The present study investigated the molecular pathology of pulmonary injury due to drowning, using lung specimens from forensic autopsy cases of drowning (n = 21), acute mechanical asphyxia due to neck compression and smothering (n = 24), and hypothermia (cold exposure, n = 11), as well as those of injury (n = 23), intoxication (n = 13), fire fatality (n = 18), and acute cardiac death (n = 9) for comparison. TaqMan real-time reverse transcription polymerase chain reaction was used to quantify messenger RNA (mRNA) expressions of pulmonary surfactant-associated proteins A and D (SP-A and SP-D), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10. SP-A and SP-D mRNA levels were lower for drowning, mechanical asphyxiation, fire fatality, and acute cardiac deaths than for hypothermia and injury. TNF-α, IL-1β, and IL-10 mRNA levels were higher for drowning or for drowning and injury than for other groups; there was no significant difference between fire fatality, involving airway injury due to inhalation of hot/irritant gases, and other control groups. These observations suggest characteristic molecular biological patterns of pulmonary injury involving suppression of pulmonary surfactants and activation of early-phase mediators of inflammation in drowning, with high mRNA expression levels of pulmonary surfactants in fatal hypothermia; however, there was no significant difference among these markers in immunohistochemical detection, except for SP-A. These mRNA expressions can be used as markers of pulmonary injury to assist in investigations of the pathophysiology of drowning and fatal hypothermia in combination with other biochemical and biological markers.