Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

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• 作者：Erzsebet Paszti-Gere (1) Gere.Erzsebet@aotk.szie.hu
  Krisztina Szeker (1)
  Edina Csibrik-Nemeth (1)
  Rita Csizinszky (1)
  Andras Marosi (1)
  Orsolya Palocz (1)
  Orsolya Farkas (1)
  Peter Galfi (1)
• 关键词：KEY WORDS IPEC ; J2 &#8211 ; probiotics &#8211 ; oxidative stress &#8211 ; proinflammatory cytokines &#8211 ; Hsp70
• 刊名：Inflammation
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：35
• 期：4
• 页码：1487-1499
• 全文大小：415.9 KB
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• 作者单位：1. Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences, Szent Istv谩n University, Istv谩n u. 2, 1078 Budapest, Hungary
• ISSN：1573-2576

文摘

Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunmodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-α level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, d- and l-lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.