Single nucleotide polymorphisms (SNPs) in key cytokines may modulate food allergy phenotypes

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• 作者：Paula Brown (1)
  Bindukumar Nair (1)
  Supriya D. Mahajan (1) smahajan@buffalo.edu
  Donald E. Sykes (1)
  Gary Rich (1)
  Jessica L. Reynolds (1)
  Ravikumar Aalinkeel (1)
  John Wheeler (1)
  Stanley A. Schwartz (1)
• 关键词：Single nucleotide polymorphisms &#8211 ; Cytokines &#8211 ; Atopy &#8211 ; Food allergy &#8211 ; RAST+ &#8211 ; IgE
• 刊名：European Food Research and Technology
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：235
• 期：5
• 页码：971-980
• 全文大小：427.9 KB
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• 作者单位：1. Division of Allergy, Immunology, and Rheumatology, Department of Medicine, University at Buffalo, 640 Ellicott Street, Room 444 Innovation Center, Buffalo Niagara Medical Campus, Buffalo, NY 14203, USA
• ISSN：1438-2385

文摘

Single nucleotide polymorphisms (SNPs) can play a direct or indirect role in phenotypic expression in food allergy pathogenesis. Our goal was to quantitate the expression of SNPs in relevant cytokines that were expressed in food allergic patients. SNPs in cytokine genes IL-4 and IL-10 are known to be important in IgE generation and regulation. We examined IL-4 (C-590T), IL-4Rα (1652A/G) and IL-10 (C-627A) SNPs using real-time PCR followed by restriction fragment length polymorphism (RFLP) analysis. Our results show that the AA, AG and GG genotypes for IL-4Rα (1652A/G) polymorphisms were statistically different in radioallergosorbent test (RAST) positive versus negative patients, and although no statistically significant differences were observed between genotypes in the IL-4 (C-590T) and IL-10 (C-627A) SNPs, we observed a significant decrease in IL-4 (C-590T) gene expression and increase in IL-4Rα (1652A/G) and IL-10 (C-627A) gene expression between RAST+ versus RAST− patients, respectively. We also observed significant modulation in the protein expression of IL-4 and IL-10 in the serum samples of the RAST+ patients as compared to the RAST− patients indicating that changes in SNP expression resulted in altered phenotypic response in these patients.