Vps1 in the late endosome-to-vacuole traffic

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• 作者：Jacob Hayden ; Michelle Williams ; Ann Granich ; Hyoeun Ahn…
• 关键词：ESCRT ; FM4 ; 64 ; HOPS ; late endosome ; traffic ; vacuole ; Vps1
• 刊名：Journal of Biosciences
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：38
• 期：1
• 页码：73-83
• 全文大小：
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• 作者单位：Jacob Hayden (1)
  Michelle Williams (1)
  Ann Granich (1)
  Hyoeun Ahn (1)
  Brandon Tenay (1)
  Joshua Lukehart (1)
  Chad Highfill (1) (2)
  Sarah Dobard (1)
  Kyoungtae Kim (1)
  
  1. Department of Biology, Missouri State University, 901 S National, Springfield, Missouri, 65807, USA
  2. Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas, 66045, USA
  
• 刊物类别：Life Sciences, general; Biomedicine general; Zoology; Plant Sciences; Microbiology; Cell Biology;
• 刊物主题：Life Sciences, general; Biomedicine general; Zoology; Plant Sciences; Microbiology; Cell Biology;
• 出版者：Springer India
• ISSN：0973-7138

文摘

Vacuolar protein sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTP hydrolyzing protein, which plays an important role in protein sorting and targeting between the Golgi and late endosomal compartments. In this study, we assessed the functional significance of Vps1 in the membrane traffic towards the vacuole. We show here that vps1螖 cells accumulated FM4-64 to a greater extent than wild-type (WT) cells, suggesting slower endocytic degradation traffic toward the vacuole. In addition, we observed that two endosome-to-vacuole traffic markers, DsRed-FYVE and Ste2-GFP, were highly accumulated in Vps1-deficient cells, further supporting Vps1鈥檚 implication in efficient trafficking of endocytosed materials to the vacuole. Noteworthy, a simultaneous imaging analysis in conjunction with FM4-64 pulse-chase experiment further revealed that Vps1 plays a role in late endosome to the vacuole transport. Consistently, our subcellular localization analysis showed that Vps1 is present at the late endosome. The hyperaccumulation of endosomal intermediates in the vps1 mutant cells appears to be caused by the disruption of integrity of HOPS tethering complexes, manifested by mislocalization of Vps39 to the cytoplasm. Finally, we postulate that Vps1 functions together with the Endosomal Sorting Complex Required for Transport (ESCRT) complex at the late endosomal compartments, based on the observation that the double mutants, in which VPS1 along with singular ESCRT I, II and III genes have been disrupted, exhibited synthetic lethality. Together, we propose that Vps1 is required for correct and efficient trafficking from the late endosomal compartments to the vacuole. Keywords ESCRT FM4-64 HOPS late endosome traffic vacuole Vps1