Rice OsPBL1 (ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1) enhanced defense of Arabidopsis against Pseudomonas syringae DC3000
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- 作者：Ekta Khare ; Kangmin Kim ; Kui-Jae Lee
- 关键词：Arabidopsis thaliana ; Defense ; Pseudomonas syringae ; Receptor ; like cytoplasmic kinases
- 刊名：European Journal of Plant Pathology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：146
- 期：4
- 页码：901-910
- 全文大小：747 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Pathology
Plant Sciences
Ecology - 出版者：Springer Netherlands
- ISSN：1573-8469
- 卷排序：146
文摘
The receptor-like cytoplasmic kinases (RLCK family VII) are required for plant defense against various pathogens. Previously, OsPBL1 (ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1) was isolated from rice as a potential RSV (rice stripe virus) resistant factor, but its physiological roles in plant defense are yet to be investigated. In this study, we demonstrated that OsPBL1increased defense against P. syringae in transgenic Arabidopsis. To ascertain the role of OsPBL1 gene in plant defense, OsPBL1 tagged with HA (i.e. Hemagglutinin) was overexpressed in Arabidopsis and examined for the resistance against Pseudomonas syringae pv. tomato DC3000 (i.e. Pst DC3000). At 3 dpi of Pst DC3000, transgenic Arabidopsis lines exhibited the reduced chlorotic lesion and propagation of P. syringae, compared to wild type. Elevated pathogen resistance of transgenic lines was correlated with increased H2O2 accumulation and callose deposition on the infected leaves. It was also revealed that expression levels of salicylic acid dependent genes such as PR1, PR2, and PR5, were induced higher in transgenic lines than wild type. Taken together, our data suggested that OsPBL1 exerted the role in defense against pathogen attacks in plant via mainly facilitating salicylic acid dependent pathway.