Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess

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• 作者：Rashmi R. Nair ; Célia M. Silveira…
• 关键词：Sulfate ; reducing bacteria ; Hydrogen cycling ; Molybdenum ; Tungsten ; Microbiologically influenced corrosion
• 刊名：Journal of Biological Inorganic Chemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：20
• 期：2
• 页码：311-322
• 全文大小：562 KB
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• 作者单位：Rashmi R. Nair (1)
  Célia M. Silveira (1)
  Mário S. Diniz (1)
  Maria G. Almeida (1)
  Jose J. G. Moura (1)
  Maria G. Rivas (1) (2)
  
  1. REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal
  2. Departamento de Física, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, S3000ZAA, Santa Fe, Argentina
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Microbiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1327

文摘

The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150?μM produce a twofold increase in the doubling time with this effect being more significant at 200?μM molybdate (five times increase in the doubling time). It was also observed that 500?μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.