Flow cytometry-assisted quantification of γH2AX expression has potential as a rapid high-throughput biodosimetry tool

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• 作者：Daniel G. Achel ; Antonio M. Serafin…
• 刊名：Radiation and Environmental Biophysics
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：55
• 期：3
• 页码：349-357
• 全文大小：750 KB
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Biophysics and Biomedical Physics
  Effects of Radiation and Radiation Protection
  Environmental Physics
  Environmental Computing and Modeling
  Ecosystems
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2099
• 卷排序：55

文摘

Large-scale radiological events require immediate and accurate estimates of doses received by victims, and possibly the first responders, to assist in treatment decisions. Although there are numerous efforts worldwide to develop biodosimetric tools to adequately handle triage needs during radiological incidents, such endeavours do not seem to actively involve sub-Saharan Africa which currently has a significant level of nuclear-related activity. To initiate a similar interest in Africa, ex vivo radiation-induced γH2AX expression in peripheral blood lymphocytes from fourteen healthy donors was assessed using flow cytometry. While the technique shows potential for use as a rapid high-throughput biodosimetric tool for radiation absorbed doses up to 5 Gy, significant inter-individual differences in γH2AX expression emerged. Also, female donors exhibited higher levels of γH2AX expression than their male counterparts. To address these shortcomings, gender-based in-house dose–response curves for γH2AX induction in lymphocytes 2, 4, and 6 h after X-ray irradiation are proposed for the South African population. The obtained results show that γH2AX is a good candidate biomarker for biodosimetry, but might need some refinement and validation through further studies involving a larger cohort of donors.KeywordsPeripheral blood lymphocytesγH2AXFlow cytometryBiodosimetry