Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi-elastic light scattering for characterization of polymersomes: comparison with classical techniques

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• 作者：Ugo Till (1) (2)
  Mireille Gaucher-Delmas (2)
  Pascale Saint-Aguet (3)
  Glenn Hamon (1)
  Jean-Daniel Marty (1)
  Christophe Chassenieux (4)
  Bruno Payré (5)
  Dominique Goudounèche (5)
  Anne-Fran?oise Mingotaud (1)
  Frédéric Violleau (2)
  
• 关键词：Asymmetrical flow field ; flow fractionation ; Polymersomes ; Self ; assemblies ; Shape ; Molecular weight
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：406
• 期：30
• 页码：7841-7853
• 全文大小：1,576 KB
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• 作者单位：Ugo Till (1) (2)
  Mireille Gaucher-Delmas (2)
  Pascale Saint-Aguet (3)
  Glenn Hamon (1)
  Jean-Daniel Marty (1)
  Christophe Chassenieux (4)
  Bruno Payré (5)
  Dominique Goudounèche (5)
  Anne-Fran?oise Mingotaud (1)
  Frédéric Violleau (2)
  
  1. Université de Toulouse, UPS/CNRS, IMRCP, 118 route de Narbonne, 31062, Toulouse Cedex 9, France
  2. Université de Toulouse, Institut National Polytechnique de Toulouse–Ecole d’Ingénieurs de Purpan, Département Sciences Agronomiques et Agroalimentaires, UPSP/DGER 115, 75 voie du TOEC, BP 57611, 31076, Toulouse Cedex 03, France
  3. Technopolym, Institut de Chimie de Toulouse, 118 route de Narbonne, 31062, Toulouse Cedex 9, France
  4. LUNAM Université, Université du Maine, IMMM UMR CNRS 6283, Département PCI, Avenue Olivier Messiaen, 72085, Le Mans Cedex 09, France
  5. Centre de Microscopie Electronique Appliquée à la Biologie, Faculté de Médecine Toulouse Rangueil, Université de Toulouse, 133, route de Narbonne, 31062, Toulouse cedex 4, France
  
• ISSN：1618-2650

文摘

Polymersomes formed from amphiphilic block copolymers, such as poly(ethyleneoxide-b-ε-caprolactone) (PEO-b-PCL) or poly(ethyleneoxide-b-methylmethacrylate), were characterized by asymmetrical flow field-flow fractionation coupled with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), and refractive index detection, leading to the determination of their size, shape, and molecular weight. The method was cross-examined with more classical ones, like batch dynamic and static light scattering, electron microscopy, and atomic force microscopy. The results show good complementarities between all the techniques; asymmetrical flow field-flow fractionation being the most pertinent one when the sample exhibits several different types of population. Figure ?/em>