Preparation of Broadly Specific Monoclonal Antibodies for Simultaneous Determination of Fluoroquinolone Residues in Eggs

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• 作者：Dapeng Peng ; Yulian Wang ; Liang Feng ; Guangcai Cao ; Yanfei Tao…
• 关键词：Fluoroquinolones ; Indirect competitive enzyme ; linked immunosorbent assay ; Monoclonal antibody ; Egg ; Residues
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：12
• 页码：3520-3531
• 全文大小：1,274 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X
• 卷排序：9

文摘

To quickly monitor fluoroquinolone (FQ) residues in eggs, three broadly specific monoclonal antibodies (mAbs; i.e., 5B11, 4B6, and 6A2) against FQs were generated. Among them, the 6A2 mAb exhibited the capability to recognize 19 FQs with equal or nearly equal efficacy and therefore was selected for the development of a simple and rapid indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The optimized ic-ELISA protocol was carried out using only acetonitrile with NaOH for the fast extraction of FQs in whole eggs. The CCα, the CCβ, and the limit of quantity values of 19 FQs in eggs ranged from 0.6 to 3.1, 0.7 to 3.6, and 1.6 to 4.2 μg kg−1, respectively. The recoveries from eggs spiked with the above FQs ranged from 60.2 to 119.9 %, with coefficients of variation (CVs) of less than 17.8 %. These results suggest that the prepared mAbs and the developed ic-ELISA protocol are reliable tools that can be applied for the screening of FQ residues in eggs.