Genetic mapping of polygenic scab (Venturia pirina) resistance in an interspecific pear family

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• 作者：Kyungho Won (1)
  Hélo?se Bastiaanse (2)
  Yoon Kyeong Kim (1)
  Jang Hoon Song (1)
  Sam Seog Kang (1)
  Han Chan Lee (1)
  Kang Hee Cho (4)
  Lester Brewer (5)
  Gagandeep Singla (2)
  Susan E. Gardiner (3)
  David Chagné (3)
  Vincent G. M. Bus (2)
  
• 关键词：Nonhost resistance ; Pyrus ; Quantitative trait loci (QTL) ; Single nucleotide polymorphism (SNP) ; Synteny
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：34
• 期：4
• 页码：2179-2189
• 全文大小：1,046 KB
• 参考文献：1. Abe K, Kotobuki K (1998a) Inheritance of high resistance to / Venturia nashicola Tanaka et Yamamoto in Japanese pear ( / Pyrus pyrifolia Nakai) and Chinese pear ( / P. ussuriensis Maxim.). J Jpn Soc Hort Sci 67:677-80 CrossRef
  2. Abe K, Kotobuki K (1998b) Polygenic inheritance of necrotic reaction to pear scab ( / Venturia nashicola Tanaka et Yamamoto) in Japanese pear ( / Pyrus pyrifolia Nakai) and Chinese pear ( / P. ussuriensis Maxim.). J Jpn Soc Hort Sci 67:839-42 CrossRef
  3. Abe K, Kotobuki K, Saito T, Terai O (2000) Inheritance of resistance to pear scab from European pears to Asian pears. J Jpn Soc Hort Sci 69:1- CrossRef
  4. Abe K, Saito T, Terai O, Sato Y, Kotobuki K (2008) Genotypic difference for the susceptibility of Japanese, Chinese and European pears to / Venturia nashicola, the cause of scab on Asian pears. Plant Breed 127:407-12 CrossRef
  5. Bell RL (1990) Pears ( / Pyrus). Acta Hort 290(2):655-97
  6. Bell RL, Quamme HA, Layne REC, Skirvin RM (1996) Pears. In: Janick J, Moore JN (eds) Fruit breeding. Volume I. Tree and tropical fruits. Wiley, Hoboken, pp 441-14
  7. Bénaouf G, Parisi L (2000) Genetics of host-pathogen relationships between / Venturia inaequalis races 6 and 7 and / Malus species. Phytopathology 90:236-42 CrossRef
  8. Bouvier L, Bourcy M, Boulay M, Tellier M, Guerif P, Denancé C, Durel C-E, Lespinasse Y (2012) A new pear scab resistance gene / Rvp1 from the European pear cultivar ‘Navara-maps in a genomic region syntenic to an apple scab resistance gene cluster on linkage group 2. Tree Genet Genom 8:53-0 CrossRef
  9. Brewer L, Alspach PA, Morgan C, Bus VGM (2009) Resistance to scab caused by / V. pirina in interspecific pear ( / Pyrus spp.) hybrids. NZ J Crop Hort Sci 37:211-18 CrossRef
  10. Broman KW (2003) Mapping quantitative trait loci in the case of a spike in the phenotype distribution. Genetics 163:1169-175
  11. Bruzzese E, Hasan S (1983) A whole leaf clearing and staining technique for host specificity studies of rust fungi. Plant Pathol 32:335-38 CrossRef
  12. Bus V, van de Weg WE, Durel CE, Gessler C, Calenge F, Parisi L, Rikkerink E, Gardiner S, Patocchi A, Meulenbroek B, Schouten H, Laurens F (2004) Delineation of a scab resistance gene cluster on linkage group 2 of apple. Acta Hort 663:57-2
  13. Bus VGM, Laurens FND, van de Weg WE, Rusholme RL, Rikkerink EHA, Gardiner SE, Bassett HCM, Kodde LP, Plummer KM (2005) The / Vh8 locus of a new gene-for-gene interaction between / Venturia inaequalis and the wild apple / Malus sieversii is closely linked to the / Vh2 locus in / Malus pumila R12740-7A. New Phytol 166:1035-049 CrossRef
  14. Bus VGM, Rikkerink EHA, Caffier V, Durel C-E, Plummer KM (2011) Revision of the nomenclature of the differential host-pathogen interactions of / Venturia inaequalis and / Malus. Ann Rev Phytopathol 49:391-13 CrossRef
  15. Bus V, Brewer L, Morgan C (2013) Observations on scab resistance in interspecific pear seedling families. Acta Hort 976:493-97
  16. Celton J-M, Chagné D, Tustin SD, Terakami S, Nishitani C, Yamamoto T, Gardiner SE (2009) Update on comparative genome mapping between / Malus and / Pyrus. BMC Res Notes 2:182 CrossRef
  17. Chagné D, Crowhurst RN, Troggio M, Davey MW, Gilmore B, Lawley C, Vanderzande S, Hellens RP, Kumar S, Cestaro A, Velasco R, Main D, Rees JD, Iezzoni A, Mockler T, Wilhelm L, Van de Weg E, Gardiner SE, Bassil N, Peace C (2012) Genome-wide SNP detection, validation, and development of an 8?K SNP array for apple. PLoS one 7(2):e31745. doi:10.1371/journal.po
• 作者单位：Kyungho Won (1)
  Hélo?se Bastiaanse (2)
  Yoon Kyeong Kim (1)
  Jang Hoon Song (1)
  Sam Seog Kang (1)
  Han Chan Lee (1)
  Kang Hee Cho (4)
  Lester Brewer (5)
  Gagandeep Singla (2)
  Susan E. Gardiner (3)
  David Chagné (3)
  Vincent G. M. Bus (2)
  
  1. National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), 121 Byeongnyugil, Geumcheon-Myeon, Naju, 520-821, Korea
  2. The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 1401, Havelock North, 4157, New Zealand
  4. National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), 475 Imok-dong, Jangan-gu, Suwon, 440-706, Korea
  5. Plant and Food Research, 55 Old Mill Rd, RD 3, Motueka, 7198, New Zealand
  3. Plant and Food Research, Private Bag 11600, Palmerston North, 4442, New Zealand
  
• ISSN：1572-9788

文摘

The need for chemical control of scab (Venturia pirina, V. nashicola) is the main barrier preventing sustainable pear production. As cultivars with durable resistance are not available yet commercially, the development of molecular markers for early selection is desirable to enhance the efficiency of breeding such cultivars. Interspecific pear progeny PEAR1?×?PEAR2 derived from European (Pyrus communis) and Asian (P. pyrifolia and P. ussuriensis) pears was infected with three single-spore isolates of V. pirina using the droplet inoculation technique. Illumina Infinium? HD Assay technology was employed to genotype the progeny with single nucleotide polymorphism markers for map construction. With one linkage group missing in each parent, the parental maps covered 17 linkage groups in total, 1,132.3 and 1,136.8 centimorgan for the female and male parents, respectively. Resistance mapping resulted in the identification of seven quantitative trait loci (QTLs) by Kruskal–Wallis analysis. Parent PEAR1 contributed a QTL on linkage group (LG) 17 that was effective against all three scab isolates, while PEAR2 contributed one on LG7 that was effective against two isolates. The other five QTLs, on LG2 and LG5 of PEAR2, and LG7 and LG10 of PEAR1, displayed differential interactions, with each QTL being paired with a single incompatible isolate. Additive effects of combined resistance loci displayed a higher level of resistance than single loci, and the role of nonhost resistance is discussed.