Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women
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- 作者：Jennifer E Balkus (1) (2)
Caroline Mitchell (2)
Kathy Agnew (2)
Congzhou Liu (3)
Tina Fiedler (3)
Susan E Cohn (4)
Amneris Luque (5)
Robert Coombs (6)
David N Fredricks (3)
Jane Hitti (2) - 关键词：Lactobacillus ; Lactobacillus crispatus ; Lactobacillus jensenii ; Bacterial vaginosis ; Culture ; Hydrogen peroxide ; 16S rRNA gene
- 刊名：BMC Infectious Diseases
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：12
- 期：1
- 全文大小：156KB
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24. The pre-publication history for this paper can be accessed here:ef="http://www.biomedcentral.com/1471-2334/12/188/prepub" class="a-plus-plus">http://www.biomedcentral.com/1471-2334/12/188/prepub - 作者单位：Jennifer E Balkus (1) (2)
Caroline Mitchell (2)
Kathy Agnew (2)
Congzhou Liu (3)
Tina Fiedler (3)
Susan E Cohn (4)
Amneris Luque (5)
Robert Coombs (6)
David N Fredricks (3)
Jane Hitti (2)
1. Department of Epidemiology, University of Washington, Box 359909, 325 9th Avenue, Seattle, WA, 98104, USA
2. Department of Obstetrics and Gynecology, University of Washington, Seattle, USA
3. Vaccine Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, USA
4. Division of Infectious Diseases, Department of Medicine, Northwestern University, Chicago, USA
5. Department of Medicine, University of Rochester, Rochester, USA
6. Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, USA
文摘
Background The presence of hydrogen peroxide (H2O2) producing Lactobacillus in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H2O2-producing Lactobacillus detected by culture with quantitative PCR (qPCR) detection of Lactobacillus species commonly assumed to be H2O2-producers. Methods Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for L. crispatus and L. jensenii using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for Lactobacillus and tested for H2O2-production. We calculated a kappa statistic to assess concordance between culture and qPCR. Results Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82%) visits, of which 233 of 308 (76%) produced H2O2. L. crispatus and/or L. jensenii were detected at 215 (57%) visits. Concordance between detection of L. crispatus and/or L. jensenii by qPCR and H2O2-producing Lactobacillus by culture was 75% (kappa = 0.45). Conclusions Among HIV-1 seropositive women, there was a moderate level of concordance between H2O2-producing Lactobacillus detected by culture and the presence of L. crispatus and/or L. jensenii by qPCR. However, one-quarter of samples with growth of H2O2-producing lactobacilli did not have L. crispatus or L. jensenii detected by qPCR. This discordance may be due to the presence of other H2O2-producing Lactobacillus species.