The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

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• 作者：Ryan W. Davis (1)
  Benjamin J. Carvalho (1)
  Howland D. T. Jones (2) (3)
  Seema Singh (1)
  
  1. Sandia National Laboratories ; Livermore ; CA ; 94551 ; USA
  2. Sandia National Laboratories ; Albuquerque ; NM ; 87185 ; USA
  3. HyperImage Solutions ; Rio Rancho ; NM ; 87144 ; USA
  
• 关键词：Microalgae ; Dunaliella viridis ; Algae bioproducts ; Semi ; continuous culture ; Photo ; osmotic adaptation ; Lipid microparticles ; Algal milking
• 刊名：Journal of Applied Phycology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：27
• 期：1
• 页码：109-123
• 全文大小：2,517 KB
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• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

Although great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, the role of interactions among these variables is largely nebulous. Here, we describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. This phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.