Liver regeneration is associated with lipid reorganization in membranes of the endoplasmic reticulum
详细信息 查看全文
- 作者：Anatoly I. Bozhkov ; Natalia G. Menzyanova ; Vadim V. Davydov…
- 关键词：regeneration ; neutral lipids ; microsomes in vitro
- 刊名：Frontiers in Biology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：11
- 期：5
- 页码：396-403
- 全文大小：1,095 KB
- 刊物主题：Proteomics; Neurobiology; Genetics and Population Dynamics; Developmental Biology; Biochemistry, general; Cell Biology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1674-7992
- 卷排序：11
文摘
BackgroundIn recent years, an adaptive endoplasmic reticulum (ER) stress response has been actively investigated. The ER membrane, isolated from the intact and regenerating liver, may be an appropriate model for investigating the association between structural and functional characteristics of ER in vivo and their corresponding behavioral characteristics in vitro. The rate of lipid synthesis and that of intracellular lipid exchange between the ER and cytosol were investigated in the intact and regenerating liver (13 h after partial hepatectomy). Particularly, membrane characteristics, surface potential, and glucose 6-phosphatase (G6Pase) activity were investigated, along with the degradation rate of G6Pase in vitro, which was estimated by the loss of G6Pase activity, formation of lipid peroxides, and size of excreted membrane vesicles.