Experimental evaluation of new chitin–chitosan graft for duraplasty

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• 作者：M. Pogorielov ; A. Kravtsova ; G. C. Reilly…
• 刊名：Journal of Materials Science: Materials in Medicine
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：28
• 期：2
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biomaterials; Biomedical Engineering; Regenerative Medicine/Tissue Engineering; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Surfaces and Interfaces, Thin Films;
• 出版者：Springer US
• ISSN：1573-4838
• 卷排序：28

文摘

Natural materials such as collagen and alginate have promising applications as dural graft substitutes. These materials are able to restore the dural defect and create optimal conditions for the development of connective tissue at the site of injury. A promising material for biomedical applications is chitosan—a linear polysaccharide obtained by the deacetylation of chitin. It has been found to be nontoxic, biodegradable, biofunctional and biocompatible in addition to having antimicrobial characteristics. In this study we designed new chitin–chitosan substitutes for dura mater closure and evaluated their effectiveness and safety. Chitosan films were produced from 3 % of chitosan (molar mass—200, 500 or 700 kDa, deacetylation rate 80–90%) with addition of 20% of chitin. Antimicrobial effictively and cell viability were analysed for the different molar masses of chitosan. The film containing chitosan of molar mass 200 kDa, had the best antimicrobial and biological activity and was successfully used for experimental duraplasty in an in vivo model. In conclusion the chitin–chitosan membrane designed here met the requirements for a dura matter graft exhibiting the ability to support cell growth, inhibit microbial growth and biodegradade at an appropriate rate. Therefore this is a promising material for clinical duroplasty.