Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates

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• 作者：Tobias Fürch ; Rajan Hollmann ; Christoph Wittmann ; Wei Wang and Wolf-Dieter Deckwer
• 关键词：Bacillus megaterium ; Metabolic flux analysis ; Central carbon pathways ; Futile cycle
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2007
• 出版时间：January, 2007
• 年：2007
• 卷：30
• 期：1
• 页码：47-59
• 全文大小：350 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Fluxes of central carbon metabolism [glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA cycle), biomass formation] were determined for several Bacillus megaterium strains (DSM319, WH320, WH323, MS941) in C- and N-limited chemostat cultures by 13C labelling experiments. The labelling patterns of proteinogenic amino acids were analysed by GC/MS and therefrom flux ratios at important nodes within the metabolic network could be calculated. On the basis of a stoichiometric metabolic model flux distributions were estimated for the different B. megaterium strains used at various cultivation conditions. Generally all strains exhibited similar metabolic flux distributions, however, several significant changes were found in (1) the glucose flux entering the PPP via the oxidative branch, (2) the reversibilities within the PPP, (3) the relative fluxes of pyruvate and acetyl-CoA fed to the TCA cycle, (4) the fluxes around the pyruvate node involving a futile cycle.