Reversing chromatin accessibility differences that distinguish homologous mitotic metaphase chromosomes
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- 作者：Wahab A. Khan ; Peter K. Rogan ; Joan H. M. Knoll
- 关键词：Chromosome decondensation ; Metaphase chromosome structure ; Super ; resolution microscopy ; DNA Topoisomerases Type II ; Fluorescence In Situ Hybridization ; Epigenetics
- 刊名：Molecular Cytogenetics
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：8
- 期：1
- 全文大小：2,175 KB
- 刊物主题：Cytogenetics; Molecular Medicine;
- 出版者：BioMed Central
- ISSN：1755-8166
文摘
Background Chromatin-modifying reagents that alter histone associating proteins, DNA conformation or its sequence are well established strategies for studying chromatin structure in interphase (G1, S, G2). Little is known about how these compounds act during metaphase. We assessed the effects of these reagents at genomic loci that show reproducible, non-random differences in accessibility to chromatin that distinguish homologous targets by single copy DNA probe fluorescence in situ hybridization (scFISH). By super-resolution 3-D structured illumination microscopy (3D-SIM) and other criteria, the differences correspond to ‘differential accessibility’ (DA) to these chromosomal regions. At these chromosomal loci, DA of the same homologous chromosome is stable and epigenetic hallmarks of less accessible interphase chromatin are present.