A novel filamentous planctomycete of the Isosphaera-Singulisphaera group isolated from a Sphagnum peat bog

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• 作者：I. S. Kulichevskaya (1)
  A. A. Ivanova (1)
  S. E. Belova (1)
  S. N. Dedysh (1) dedysh@mail.ru
• 关键词：planctomycetes – ; filamentous forms – ; wetlands – ; Singulisphaera – ; “ ; Nostocoida”
• 刊名：Microbiology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：81
• 期：4
• 页码：446-452
• 全文大小：697.7 KB
• 参考文献：1. Schlesner, H. and Stackebrandt, E., Assignment of the Genera Planctomyces and Pirella to a New Family Planctomycetaceae fam. nov., and Description of the Order Planctomycetales ord. nov., Syst. Appl. Microbiol., 1986, vol. 8, pp. 174–176.
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  4. Kulichevskaya, I.S., Pankratov, T.A., and Dedysh, S.N., Detection of Representatives of the Planctomycetes in Sphagnum Peat Bogs by Molecular and Cultivation Approaches, Microbiology, 2006, vol. 75, no. 3, pp. 329–335.
  5. Ivanova, A.O. and Dedysh, S.N., High Abundance of Planctomycetes in Anoxic Layers of a Sphagnum Peat Bog, Microbiology, 2006, vol. 75, no. 6, pp. 716–719.
  6. Dedysh, S.N., Pankratov, T.A., Belova, S.E., Kulichevskaya, I.S., and Liesack, W., Phylogenetic Analysis and in situ Identification of Bacteria Community Composition in an Acidic Sphagnum Peat Bog, Appl. Environ. Microbiol., 2006, vol. 72, no. 3, pp. 2110–2117.
  7. Ivanova, A.O. and Dedysh, S.N., Abundance, Diversity and Depth Distribution of Planctomycetes in Acidic Northern Wetlands, Frontiers Microbiol., 2012. doi: 10.3389/fmicb.2012.00005
  8. Kulichevskaya, I.S., Ivanova, A.O., Belova, S.E., Baulina, O.I., Bodelier, P.L.E., Rijpstra, W.I.C., Sinninghe Damst茅, J.S., Zavarzin, G.A., and Dedysh, S.N., Schlesneria paludicola gen. nov., sp. nov., the First Acidophilic Member of the Order Planctomycetales, from Sphagnum-Dominated Boreal Wetlands, Int. J. Syst. Evol. Microbiol., 2007, vol. 57, pp. 2680–2687.
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  10. Kulichevskaya, I.S., Baulina, O.I., Bodelier, P.L.E., Rijpstra, W.I.C., Sinninghe Damst茅, G.S., and Dedysh, S.N., Zavarzinella formosa gen. nov., sp. nov., a Novel Stalked, Gemmata-Like Planctomycete from a Siberian Peat Bog, Int. J. Syst. Evol. Microbiol., 2009, vol. 59, pp. 357–364.
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• 作者单位：1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312 Russia
• ISSN：1608-3237

文摘

Planctomycetes are common inhabitants of northern wetlands. A significant proportion of these bacteria revealed in peat with the Planctomycetes-specific probes PLA46 and PLA886 is represented by filamentous forms which have not been cultured under laboratory conditions. In the present work, one of such organisms was isolated from a Sphagnum peat bog in a monoculture. The organism had large spherical cells assembled in long filaments. It could grow only in associations with bacterial satellites; attempts to isolate it in pure culture were unsuccessful. The organism was identified by PCR amplification, cloning, and subsequent analysis of its 16S rRNA gene fragment. Comparative sequence analysis revealed its affiliation with the Isosphaera-Singulisphaera group within the order Planctomycetales. The nucleotide sequence of the 16S rRNA gene of the new organism exhibited 94–96% similarity to those of the unicellular peat-inhabiting planctomycete Singulisphaera acidiphila and uncharacterized filamentous planctomycete “Nostocoida limicola III” from activated sludge. The new planctomycete utilized heteropolysaccharides of microbial origin as growth substrates and could grow at the low pH and temperatures typical of the northern wetlands.