Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing
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  • 作者：Yi-Bin Lu (1)
    Lin-Tong Yang (1) (2)
    Yi-Ping Qi (3)
    Yan Li (1)
    Zhong Li (1)
    Yan-Bin Chen (1)
    Zeng-Rong Huang (1)
    Li-Song Chen (1) (2) (4)

    1. College of Resources and Environmental Sciences     ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
    2. Institute of Horticultural Plant Physiology     ; Biochemistry and Molecular Biology ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
    3. Institute of Materia Medica     ; Fujian Academy of Medical Sciences ; Fuzhou ; 350001 ; China
    4. Fujian Key Laboratory for Plant Molecular and Cell Biology     ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
  • 关键词：Boron ; deficiency ; Boron ; tolerance ; Citrus sinensis ; Illumina sequencing ; microRNA ; Reactive oxygen species
  • 刊名：BMC Plant Biology
  • 出版年：2014
  • 出版时间：December 2014
  • 年：2014
  • 卷：14
  • 期：1
  • 全文大小：545 KB
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  • 刊物主题：Plant Sciences; Agriculture; Tree Biology;
  • 出版者：BioMed Central
  • ISSN：1471-2229
文摘
Background Boron (B)-deficiency is a widespread problem in many crops, including Citrus. MicroRNAs (miRNAs) play important roles in nutrient deficiencies. However, little is known on B-deficiency-responsive miRNAs in plants. In this study, we first identified miRNAs and their expression pattern in B-deficient Citrus sinensis roots by Illumina sequencing in order to identify miRNAs that might be involved in the tolerance of plants to B-deficiency. Results We isolated 52 (40 known and 12 novel) up-regulated and 82 (72 known and 10 novel) down-regulated miRNAs from B-deficient roots, demonstrating remarkable metabolic flexibility of roots, which might contribute to the tolerance of plants to B-deficiency. A model for the possible roles of miRNAs in the tolerance of roots to B-deficiency was proposed. miRNAs might regulate the adaptations of roots to B-deficiency through following several aspects: (a) inactivating reactive oxygen species (ROS) signaling and scavenging through up-regulating miR474 and down-regulating miR782 and miR843; (b) increasing lateral root number by lowering miR5023 expression and maintaining a certain phenotype favorable for B-deficiency-tolerance by increasing miR394 expression; (c) enhancing cell transport by decreasing the transcripts of miR830, miR5266 and miR3465; (d) improving osmoprotection (miR474) and regulating other metabolic reactions (miR5023 and miR821). Other miRNAs such as miR472 and miR2118 in roots increased in response to B-deficiency, thus decreasing the expression of their target genes, which are involved in disease resistance, and hence, the disease resistance of roots. Conclusions Our work demonstrates the possible roles of miRNAs and related mechanisms in the response of plant roots to B-deficiency.

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