Biodegradation of low concentrations of 1,2-dibromoethane in groundwater is enhanced by phenol

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• 作者：Kyunghwa Baek (1)
  Meng Wang (2)
  Robert McKeever (2)
  Kahlil Rieber (1)
  Chul Park (2)
  Klaus Nüsslein (1)
  
• 关键词：Biostimulation ; Cometabolism ; 1 ; 2 ; dibromoethane ; Groundwater ; Natural attenuation ; Phenol
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：98
• 期：3
• 页码：1329-1338
• 全文大小：457 KB
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• 作者单位：Kyunghwa Baek (1)
  Meng Wang (2)
  Robert McKeever (2)
  Kahlil Rieber (1)
  Chul Park (2)
  Klaus Nüsslein (1)
  
  1. Department of Microbiology, University of Massachusetts, Amherst, MA, 01003, USA
  2. Department of Civil & Environmental Engineering, University of Massachusetts, Amherst, MA, 01003, USA
  
• ISSN：1432-0614

文摘

The lead scavenger 1,2-dibromoethane (EDB), a former additive to leaded gasoline, is a common groundwater contaminant, yet not much knowledge is available for its targeted bioremediation, especially under in situ conditions. The study site was an aviation gas spill site, which, although all hydrocarbons and most of the EDB were remediated in the mid-1990s, still exhibits low levels of EDB remaining in the groundwater (about 11?μg EDB/l). To evaluate the effect of phenol on biostimulation of low concentration of EDB, microcosms were established from an EDB-contaminated aquifer. After 300?days at environmentally relevant conditions (12?±-?°C, static incubation), EDB was not significantly removed from unamended microcosms compared to the abiotic control. However, in treatments amended with phenol, up to 80?% of the initial EDB concentration had been degraded, while added phenol was removed completely. Microbial community composition in unamended and phenol-amended microcosms remained unchanged, and Polaromonas sp. dominated both types of microcosms, but total bacterial abundance and numbers of the gene for phenol hydroxylase were higher in phenol-amended microcosms. Dehalogenase, an indicator suggesting targeted aerobic biodegradation of EDB, was not detected in either treatment. This finding suggests phenol hydroxylase, rather than a dehalogenation reaction, may be responsible for 1,2-dibromoethane oxidation under in situ conditions. In addition, biostimulation of EDB is possible through the addition of low levels of phenol in aerobic groundwater sites.