Changes of chlorine isotope composition characterize bacterial dehalogenation of dichloromethane

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• 作者：A. M. Zyakun ; Yu. E. Firsova ; M. L. Torgonskaya ; N. V. Doronina and Yu. A. Trotsenko
• 刊名：Applied Biochemistry and Microbiology
• 出版年：2007
• 出版时间：November, 2007
• 年：2007
• 卷：43
• 期：6
• 页码：593-597
• 全文大小：175.1 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3024

文摘

Fractionation of dichloromethane (DCM) molecules with different chlorine isotopes by aerobic methylobacteria Methylobacterium dichloromethanicum DM4 and Albibacter methylovorans DM10; cell-free extract of strain DM4; and transconjugant Iethylobacterium extorquens AI1/pME 8220, expressing the dcmA gene for DCM dehalogenase but unable to grow on DCM, was studied. Kinetic indices of DCM isotopomers for chlorine during bacterial dehalogenation and diffusion were compared. A two-step model is proposed, which suggests diffusional DCM transport to bacterial cells.