Heterologous expression of the dehydrin-like protein gene AmCIP from Ammopiptanthus mongolicus enhances viability of Escherichia coli and tobacco under cold stress

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• 作者：Jing Shi ; Meiqin Liu ; Yuzhen Chen ; Jinyu Wang ; Cunfu Lu
• 关键词：Ammopiptanthus mongolicus ; AmCIP ; Dehydrin ; Cryoprotective function
• 刊名：Plant Growth Regulation
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：79
• 期：1
• 页码：71-80
• 全文大小：1,584 KB
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• 作者单位：Jing Shi (1)
  Meiqin Liu (1)
  Yuzhen Chen (1)
  Jinyu Wang (2)
  Cunfu Lu (1)
  
  1. College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China
  2. Analysis and Testing Center, Tsinghua University, Beijing, 100083, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

Ammopiptanthus mongolicus is the only evergreen broadleaf shrub endemic to the Alashan Desert in northwestern China. The plants can survive temperatures of −30 °C or less in winter. A dehydrin-like protein gene, AmCIP, cloned from a cold-acclimated A. mongolicus seedling was transformed into E. coli. The transgenic strains exhibited enhanced freezing tolerance compared with non-transformed host cells. The recombinant AmCIP remained soluble pre- and post-boiling for 10 min, and protected activity of lactate dehydrogenase during two freeze–thaw cycles more effectively than bovine serum albumin, a protein with a proven cryoprotective effect. Expression of AmCIP in transgenic tobacco increased cold tolerance during seed germination and seedling growth. The YFP-AmCIP fusion protein was localized in the cytoplasm and nucleus in onion inner epidermal cells, indicating that AmCIP might function both in the cytoplasm and nucleus.