Production of Cellobiose Dehydrogenase from a Newly Isolated White Rot Fungus Termitomyces sp. OE147

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• 作者：Gupteshwar Gupta (1)
  Rishabh Gangwar (1)
  Ashwani Gautam (1)
  Lalit Kumar (1)
  Anuj Dhariwal (2)
  Vikram Sahai (1)
  Saroj Mishra (1)
  
• 关键词：Termitomyces sp. OE 147 ; Cellobiose dehydrogenase ; Lactobionic acid ; Optimized Ludwig medium
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：173
• 期：8
• 页码：2099-2115
• 全文大小：648 KB
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• 作者单位：Gupteshwar Gupta (1)
  Rishabh Gangwar (1)
  Ashwani Gautam (1)
  Lalit Kumar (1)
  Anuj Dhariwal (2)
  Vikram Sahai (1)
  Saroj Mishra (1)
  
  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, 110016, India
  2. JAIV Technologies Pvt., Ltd., Sector 10 Dwarka, New Delhi, 110075, India
  
• ISSN：1559-0291

文摘

Class I cellobiose dehydrogenases (CDHs) are extracellular hemoflavo enzymes produced at low levels by the Basidiomycetes (white rot fungi). In presence of suitable electron acceptors, e.g., cytochrome c, 2,6-dichlorophenol-indophenol, or metal ions, it oxidizes cellobiose to cellobionolactone. A stringent requirement for disaccharides makes CDH also useful for conversion of lactose to lactobionic acid, an important ingredient in pharma and detergent industry. In this work, class I CDH was produced using a newly identified white rot fungus Termitomyces sp. OE147. Four media were evaluated for CDH production, and maximum enzyme activity of 0.92 international unit (IU)/ml was obtained on Ludwig medium under submerged conditions. Statistical optimization of N source, which had significant effect on CDH production, using Box–Behnken design followed by optimization of inoculum size and age resulted in an increase in activity to 2.9?IU/ml and a productivity of ~25?IU/l/h. The nearly purified CDH exhibited high activity of 26.4?IU/mg protein on lactose indicating this enzyme to be useful for lactobionic acid synthesis. Some of the internal peptide sequences bore 100?% homology to the CDH produced in Myceliophthora thermophila. The fungal isolate was amenable to scale up, and an overall productivity of ~18?IU/l/h was obtained at 14-l level.