Ethanol content in plants of Brassica napus L. correlated with waterlogging tolerance index and regulated by lactate dehydrogenase and citrate synthase

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• 作者：Benbo Xu ; Yong Cheng ; Xiling Zou ; Xuekun Zhang
• 关键词：Alcohol dehydrogenase ; Glyceraldehyde ; 3 ; phosphate dehydrogenase ; Citrate synthase ; Lactate dehydrogenase ; Pyruvate decarboxylase ; Waterlogging tolerance index
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：38
• 期：3
• 全文大小：890 KB
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• 作者单位：Benbo Xu (1) (2)
  Yong Cheng (2)
  Xiling Zou (2)
  Xuekun Zhang (2)
  
  1. College of Life Sciences, Yangtze University, Jingzhou, 434025, Hubei, People’s Republic of China
  2. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, Hubei, People’s Republic of China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Oilseed rape (Brassica napus L.) is susceptible to waterlogging stress. To study the role of some genes on waterlogging tolerance in B. napus, the ethanol content and expression levels of some key genes involved in both anaerobic and aerobic metabolism were studied in 12 cultivars of B. napus with different degrees of waterlogging tolerance. The waterlogging tolerance index (WTI) was significantly negatively correlated with the ethanol content and the transcript abundance of the lactate dehydrogenase (LDH) gene, and significantly positively correlated with transcript level of the citrate synthase (CTS) gene. WTI was not correlated with the transcript levels of genes encoding pyruvate decarboxylase (PDC), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), or alcohol dehydrogenase (ADH). The correlation coefficient between WTI and ethanol content at 24 and 48 h after waterlogging treatments was 0.82 and 0.60, respectively. The correlation coefficient between WTI and the transcript level of BnCTS at 24 h and BnLDH at 48 h after waterlogging treatments was 0.74 and 0.73, respectively. These findings suggest that improving the O₂ uptaking and transporting ability is important for increasing plant tolerance to waterlogging stress. Cultivars with strong waterlogging tolerance can be selected based on their ethanol content at 24 h after waterlogging treatment.