The isozyme spectra of lactate dehydrogenase in the tissues of the raccoon dog Nyctereutes procyonoides in the autumn

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• 作者：A. R. Unzhakov ; N. N. Tyutyunnik
• 关键词：lactate dehydrogenase ; isozymes ; autumn ; raccoon dog ; red fox ; Arctic blue fox
• 刊名：Biophysics
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：61
• 期：4
• 页码：640-646
• 全文大小：350 KB
• 刊物主题：Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1555-6654
• 卷排序：61

文摘

An electrophoretic assay of lactate dehydrogenase (EC 1.1.1.27) isozymes in the tissue homogenates of cardiac and skeletal muscles, kidney, lungs, spleen, and liver of the raccoon dog Nyctereutes procyonoides from two different geographic zones, viz., northwestern Russia and Poland, as well as the Arctic blue fox Alopex lagopus L. and the red fox Vulpes vulpes L. was performed during the preparatory period to the winter season. Raccoon dogs, which hibernate under natural conditions, differ from other canids (the red fox and Arctic blue fox) to which they are close taxonomically by their body weight and by the higher proportion of aerobic H subunits of lactate dehydrogenase in all organs except for the heart. A higher content of “fast” anode fractions, lactate dehydrogenase-1 and lactate dehydrogenase-2, in the heart, kidney, lungs, liver, and spleen was detected in the raccoon dogs from the northern region compared to those from the southern geographic zone. The shift in the reaction catalyzed by lactate dehydrogenase towards the production of pyruvate indicates that this metabolite is necessary for the synthesis of fatty acids during lipogenesis in the autumn.