An alternative high output tissue microarray technique

详细信息    查看全文

• 作者：Yuan Shi (1)
  Deming He (1)
  Yingyong Hou (1)
  Qin Hu (1)
  Chen Xu (1)
  Yalan Liu (1)
  Dongxian Jiang (1)
  Jieakesu Su (1)
  Haiying Zeng (1)
  Yunshan Tan (1)
  
• 关键词：Tissue rod ; Tissue microarray ; Technology ; Sampling tool ; High output
• 刊名：Diagnostic Pathology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：8
• 期：1
• 全文大小：414KB
• 参考文献：1. Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. / Lab Invest 1986,55(2):244-48.
  2. Wan WH, Fortuna MB, Furmanski P: A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously. / J Immunol Methods 1987,103(1):121-29. CrossRef
  3. Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. / Nat Med 1998,4(7):844-47. CrossRef
  4. Jawhar NM: Tissue microarray: a rapidly evolving diagnostic and research tool. / Ann Saudi Med 2009,29(2):123-27. CrossRef
  5. Avninder S, Ylaya K, Hewitt SM: Tissue microarray: a simple technology that has revolutionized research in pathology. / J Postgrad Med 2008,54(2):158-62. CrossRef
  6. Faratian D, Graham A, Rae F, Thomas J: Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service. / Histopathology 2009,54(4):428-32. CrossRef
  7. Schraml P, Kononen J, Bubendorf L, Moch H, Bissig H, Nocito A, Mihatsch MJ, Kallioniemi OP, Sauter G: Tissue microarrays for gene amplification surveys in many different tumor types. / Clin Cancer Res 1999,5(8):1966-975.
  8. Veeck J, Dahl E: RNA expression analysis on formalin-fixed paraffin-embedded tissues in TMA format by RNA in situ hybridization. / Methods Mol Biol 2010, 664:135-50. CrossRef
  9. Schlomm T, Chun F, Erbersdobler A: From gene to clinic: TMA-based clinical validation of molecular markers in prostate cancer. / Methods Mol Biol 2010, 664:177-89. CrossRef
  10. Kallioniemi A: DNA copy number analysis on tissue microarrays. / Methods Mol Biol 2010, 664:127-34. CrossRef
  11. Gulmann C, Loring P, O'Grady A, Kay E: Miniature tissue microarrays for HercepTest standardisation and analysis. / J Clin Pathol 2004,57(11):1229-231. CrossRef
  12. Gately K, Kerr K, O'Byrne K: Design, construction, and analysis of cell line arrays and tissue microarrays for gene expression analysis. / Methods Mol Biol 2011, 784:139-53. CrossRef
  13. Shebl AM, Zalata KR, Amin MM, El-Hawary AK: An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips. / Diagn Pathol 2011, 6:117. CrossRef
  14. Fowler CB, Man YG, Zhang S, O'Leary TJ, Mason JT, Cunningham RE: Tissue microarrays: construction and uses. / Methods Mol Biol 2011, 724:23-5. CrossRef
  15. Rubin BP, Singer S, Tsao C, Duensing A, Lux ML, Ruiz R, Hibbard MK, Chen CJ, Xiao S, Tuveson DA, and others: KIT activation is a ubiquitous feature of gastrointestinal stromal tumors. / Cancer Res 2001,61(22):8118-121.
  16. Grabellus F, Ebeling P, Worm K, Sheu SY, Antoch G, Frilling A, Schmid KW: Double resistance to imatinib and AMG 706 caused by multiple acquired KIT exon 17 mutations in a gastrointestinal stromal tumour. / Gut 2007,56(7):1025-026. CrossRef
  17. Hou YY, Tan YS, Xu JF, Wang XN, Lu SH, Ji Y, Wang J, Zhu XZ: Schwannoma of the gastrointestinal tract: a clinicopathological, immunohistochemical and ultrastructural study of 33 cases. / Histopathology 2006,48(5):536-45. CrossRef
  18. Pires AR, Andreiuolo Fda M, De Souza SR: TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles. / Diagn Pathol 2006, 1:14. CrossRef
  19. Jhavar S, Corbishley CM, Dearnaley D, Fisher C, Falconer A, Parker C, Eeles R, Cooper CS: Construction of tissue microarrays from prostate needle biopsy specimens. / Br J Cancer 2005,93(4):478-82. CrossRef
  20. Vogel UF: The construction of high-density paraffin tissue microarrays with 0.43-mm-diameter paraffin tissue core biopsies is technically feasible. / Virchows Arch 2008,453(1):43-6. CrossRef
  21. Mucci NR, Akdas G, Manely S, Rubin MA: Neuroendocrine expression in metastatic prostate cancer: evaluation of high throughput tissue microarrays to detect heterogeneous protein expression. / Hum Pathol 2000,31(4):406-14. CrossRef
  22. Hoos A, Cordon-Cardo C: Tissue microarray profiling of cancer specimens and cell lines: opportunities and limitations. / Lab Invest 2001,81(10):1331-338. CrossRef
  23. Mengel M, Kreipe H, von Wasielewski R: Rapid and large-scale transition of new tumor biomarkers to clinical biopsy material by innovative tissue microarray systems. / Appl Immunohistochem Mol Morphol 2003,11(3):261-68. CrossRef
  24. Miettinen M: A simple method for generating multitissue blocks without special equipment. / Appl Immunohistochem Mol Morphol 2012,20(4):410-12. CrossRef
  25. Battifora H, Mehta P: The checkerboard tissue block. An improved multitissue control block. / Lab Invest 1990,63(5):722-24.
  
• 作者单位：Yuan Shi (1)
  Deming He (1)
  Yingyong Hou (1)
  Qin Hu (1)
  Chen Xu (1)
  Yalan Liu (1)
  Dongxian Jiang (1)
  Jieakesu Su (1)
  Haiying Zeng (1)
  Yunshan Tan (1)
  
  1. Department of Pathology, Zhongshan Hospital, Fudan University, Fenglin Road #180, 200032, Shanghai, P.R. China
  

文摘

Background Tissue microarray (TMA) is a high throughput research tool, which has greatly facilitated and accelerated in situ tissue analyses. However, its productivity has been restricted due to the confined thickness of traditional donor block. Here, we introduce an improved high output TMA method that is applicable to a broader range of tissue samples. Methods In this method, a 3.6 cm long and 2.7 cm wide recipient block with 88 square lattices (3 mm in width) was first prepared using several commercial instruments. A 2 mm wide and 6 mm long tissue rod was then prepared using a self-made blade-shaped knife from each paraffin embedded donor block of gastrointestinal stromal tumors. These rods were manually arrayed one by one into the corresponding lattices of the 60°C pre-softened recipient block with the guide of holes drilled with a steel needle. A 70-rod TMA was made to testify this method. Results The prepared TMA had well defined array configurations, good tissue morphology and fully preserved proteins and DNA. A total of 500-000 TMA sections could be easily obtained from a TMA block. Conclusion This low-cost and time-saving method provides an alternative sampling tool for high output TMA. Virtual Slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1979605867857990