Expression and Distribution of Laminin Receptor Precursor/Laminin Receptor in Rabbit Tissues

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• 作者：Huinuan Wang (1)
  Lifeng Yang (1)
  Mohammed Kouadir (1)
  Rongrong Tan (1)
  Wenyu Wu (1)
  Huarong Zou (1)
  Jin Wang (1)
  Sher Hayat Khan (1)
  Dongfeng Li (2)
  Xiangmei Zhou (1)
  Xiaomin Yin (1)
  Yunsheng Wang (1)
  Deming Zhao (1)
  
• 关键词：Laminin receptor precursor/laminin receptor ; Rabbit ; Expression ; Distribution ; Prion
• 刊名：Journal of Molecular Neuroscience
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：51
• 期：2
• 页码：591-601
• 全文大小：
• 作者单位：Huinuan Wang (1)
  Lifeng Yang (1)
  Mohammed Kouadir (1)
  Rongrong Tan (1)
  Wenyu Wu (1)
  Huarong Zou (1)
  Jin Wang (1)
  Sher Hayat Khan (1)
  Dongfeng Li (2)
  Xiangmei Zhou (1)
  Xiaomin Yin (1)
  Yunsheng Wang (1)
  Deming Zhao (1)
  
  1. State Key Laboratories for Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China
  2. National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
  
• ISSN：1559-1166

文摘

The 37/67-kDa laminin receptor precursor (LRP)/laminin receptor (LR) is a cell surface receptor for cellular prion proteins and misfolded pathological prions. Previous research has shown that blocking or decreasing LRP/LP levels by anti-LRP/LR antibodies or small interfering RNAs (siRNAs) can prolong the incubation phase of experimental prion infection. This study aimed to investigate potential mechanisms contributing to prion resistance/susceptibility by using the rabbit, a species unsusceptible to prion infection, as a model. We investigated the expression level and distribution of LRP/LR in rabbit tissues by real-time polymerase chain reaction and by immunochemical analysis with a monoclonal anti-67?kDa LR antibody. Our results showed LRP/LR mRNA expression in all the tissues examined. Very low LRP/LR expression levels were observed in central nervous system (CNS) tissues, whereas high expression levels were observed in reproductive and digestive tissues, which differed from the expression patterns that have been reported for prion-susceptible animals. The immunochemical staining results were generally consistent with the mRNA findings, although no LR protein was detected in CNS tissues. Our findings provide a basis for further studies on prion resistance in rabbits and other animal species.