Oral Antibiotic Treatment Induces Skin Microbiota Dysbiosis and Influences Wound Healing

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• 作者：Meiling Zhang ; Ziwei Jiang ; Dongqing Li ; Deming Jiang ; Yelin Wu…
• 关键词：Vancomycin ; Wound healing ; Skin microbiota ; RegIIIγ
• 刊名：Microbial Ecology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：69
• 期：2
• 页码：415-421
• 全文大小：585 KB
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  15. Garc
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Ecology
  Geoecology and Natural Processes
  Nature Conservation
  
• 出版者：Springer New York
• ISSN：1432-184X

文摘

Antibiotic treatment eliminates commensal bacteria and impairs mucosal innate immune defenses in the gut. However, whether oral antibiotic treatment could alter the composition of the microbiota on the skin surface and influence innate immune responses remains unclear. To test this, mice were treated with vancomycin for 7?days and then wounds were made on the back skin of the mice. Five days later, scar tissue from each mouse was collected for bacterial enumeration, the bacterial composition on the scar and unwounded skin was determined using 16S RNA gene-based pyrosequencing analysis, and skin around wounds was collected for RNA extraction. Compared with the control group, the overall density and composition of skin bacteria were altered, and the proportion of Staphylococcus-related sequences was reduced in the vancomycin-treated group. Moreover, vancomycin treatment decreased the expression of RegIIIγ and interleukin (IL)-17 in the wounded skin. Taken together, our data demonstrate that antibiotic treatment decreases the bacterial density and alters the bacterial composition in skin wounds, followed by a decrease in RegIIIγ expression, which may contribute to the delayed wound repair. Our findings also indicate that antibiotic therapy should be carefully considered in the treatment of skin injury.