Toxicity of the azo dyes Acid Red 97 and Bismarck Brown Y to Western clawed frog (Silurana tropicalis)

详细信息    查看全文

• 作者：Jeriel J. Soriano (1)
  Justine Mathieu-Denoncourt (1)
  Grant Norman (1)
  Shane R. de Solla (2)
  Valérie S. Langlois (1)
  
• 关键词：Silurana tropicalis ; Malformations ; Oxidative stress ; Heat shock proteins ; Tumor ; suppressing protein p53 ; Androgen receptor ; 5 Alpha ; reductase ; Steroidogenic acute regulatory protein
• 刊名：Environmental Science and Pollution Research
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：21
• 期：5
• 页码：3582-3591
• 全文大小：492 KB
• 作者单位：Jeriel J. Soriano (1)
  Justine Mathieu-Denoncourt (1)
  Grant Norman (1)
  Shane R. de Solla (2)
  Valérie S. Langlois (1)
  
  1. Chemistry and Chemical Engineering Department, Royal Military College of Canada, P. O. Box 17000, Stn Forces, Kingston, ON, Canada, K7K 7B4
  2. Wildlife and Landscape Science Directorate, Environment Canada, Burlington, ON, Canada, L7R 4A6
  
• ISSN：1614-7499

文摘

Azo compounds are used in a variety of industrial applications, such as textile colorant. Azo dyes have been found to contaminate aquatic environments and it has been shown that these compounds could potentially be toxic or induce endocrine disruption in aquatic organisms. However, there are few data available on the toxicity of these dyes, specifically Acid Red 97 (AR97) and Bismarck Brown Y (BBY). The aim of this study was to determine the toxicity and the endocrine-disrupting properties of AR97 and BBY in frogs. As fugacity modeling predicted that both compounds would sorb to sediment, sediment exposures were performed using a geometric range of concentrations (0, 1, 10, 100 and 1,000?ppm). Both AR97 and BBY dyes were not lethal to Silurana tropicalis embryos; however, BBY significantly induced malformations. Gene expression analysis of oxidative stress and mutagen-related genes was performed in BBY-treated larvae. There were significant two-fold increases of the tumor-suppressing protein p53 and heat shock protein 70 mRNA at 1,000?ppm suggesting that BBY induces cellular stress in early S. tropicalis development. Transcripts of the heat shock protein 90 did not change. Furthermore, reproductive-related genes were assessed and a 2.1-fold change was observed in the mRNA of the steroidogenic acute regulatory protein while steroid 5 alpha-reductase type 2 and androgen receptor transcript levels did not vary among treatments. In conclusion, high concentrations of BBY lead to increased developmental defects in frog embryogenesis and early larval development.