Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses
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- 作者：Yu-Jin Kim (1)
Moon-Gi Jang (1)
Hye-Jin Lee (1)
Gyu-Hwan Jang (1)
Johan Sukweenadhi (1)
Woo-Saeng Kwon (1)
Deok-Chun Yang (1) - 关键词：BetV1 protein ; Biotic stress ; Panax ginseng ; Pathogenesis ; related protein
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：118
- 期：3
- 页码：531-543
- 全文大小：2,157 KB
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Moon-Gi Jang (1)
Hye-Jin Lee (1)
Gyu-Hwan Jang (1)
Johan Sukweenadhi (1)
Woo-Saeng Kwon (1)
Deok-Chun Yang (1)
1. Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, Suwon, 449-701, Korea - ISSN：1573-5044
文摘
Pathogenesis-related proteins (PRs) are known to function in higher plants as a protein-based defensive system against abiotic and biotic stress, particularly pathogen infections. A full-length cDNA sequence of PR BetV1 was isolated and characterized from a 14-year-old ginseng expressed sequence tags library and we named this as PgPR10-4, because of similar identities with previous isolated PgPR10s sequences. The PgPR10-4 gene encodes a 477?bp open reading frame and its deduced protein contains 158 amino acids with a 53?% identity with that of the Actinidia chinensis BetV1 allergen. The expression of PgPR10-4 gene was abundant in leaves and its transcripts showed differentially up-regulated patterns against several ginseng pathogens and abiotic stimuli such as high light and salinity. In addition, PgPR10-4 expression was strongly responsive towards the stress signaling molecules H2O2 and jasmonic acid (JA), while weekly responsive to salicylic acid and abscisic acid. A functional role of PgPR10-4 in environmental stress tolerance was further validated through its overexpression in Arabidopsis. An analysis of T2 transgenic Arabidopsis plants overexpressing the PgPR10-4 gene showed an enhanced tolerance to bacterial and fungal infection, but not to salt stress. When we tagged with cyan fluorescent protein fusion protein, the PgPR10-4-was found to localize to the cytoplasm. The enhanced antifungal activity observed from the Arabidopsis transgenic lines suggests the possible involvement of PgPR10-4 in a defense-related mechanism via the JA signaling pathway.