Effect of 4-week feeding of deoxynivalenol- or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.)

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• 作者：Csilla Pelyhe ; Benjámin Kövesi ; Erika Zándoki ; Balázs Kovács…
• 关键词：Deoxynivalenol ; T ; 2 toxin ; Lipid peroxidation ; Glutathione ; Common carp
• 刊名：Mycotoxin Research
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：32
• 期：2
• 页码：77-83
• 全文大小：304 KB
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• 作者单位：Csilla Pelyhe (1)
  Benjámin Kövesi (1)
  Erika Zándoki (2)
  Balázs Kovács (3)
  Judit Szabó-Fodor (2)
  Miklós Mézes (1)
  Krisztián Balogh (1)
  
  1. Department of Nutrition, Szent István University, 2103, Gödöllő, Hungary
  2. Mycotoxins in the Food Chain Research Group, Hungarian Academy of Sciences-Kaposvár University, 7400, Kaposvár, Hungary
  3. Department of Aquaculture, Szent István University, 2103, Gödöllő, Hungary
  
• 刊物主题：Microbiology; Medical Microbiology; Chemistry/Food Science, general; Medicine/Public Health, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1867-1632

文摘

The purpose of study was to investigate the effects of T-2 toxin (4.11 mg T-2 toxin and 0.45 mg HT-2 toxin kg−1 feed) and deoxynivalenol (5.96 and 0.33 mg 15-acetyl deoxynivalenol (DON) kg−1 feed) in 1-year-old common carp juveniles in a 4-week feeding trial. The exposure of mycotoxins resulted in increased mortality in both groups consuming mycotoxin-contaminated diet. Parameters of lipid peroxidation were not affected during the trial, and antioxidant defence also did not show response to oxidative stress; however, glutatione peroxidase activity slightly, but significantly, decreased in the T-2 toxin group. Glutathione S-transferase activity showed moderate decrease as effect of T-2 toxin, which suggests its effect on xenobiotic transformation. Reduced glutathione concentration showed moderate changes as effect of DON exposure, but T-2 toxin has no effect. Expression of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes showed different response to mycotoxin exposure. T-2 toxin caused dual response in the expression of gpx4a (early and late downregulation and mid-term upregulation), but continuous upregulation was found as effect of deoxynivalenol. Expression of the other gene, gpx4b, was upregulated by both trichothecenes during the whole period. The results suggested that trichothecenes have some effect on free radical formation and antioxidant defence, but the changes depend on the duration of exposure and the dose applied, and in case of glutathione peroxidase, there was no correlation between expression of genes and enzyme activity.