Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis

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• 作者：Isaac Queiroz de Oliveira (1)
  Rodrigo Araujo Silva (1)
  Michel Vergne Sucupira (2)
  Edmilson Domingos da Silva (2)
  Alexandre Barbosa Reis (3) (4)
  Gabriel Grimaldi Jr (1)
  Deborah Bittencourt Moth茅 Fraga (1) (3) (5)
  Patr铆cia Sampaio Tavares Veras (1) (3)
  
  1. Laborat贸rio de Patologia e Biointerven莽茫o ; Centro de Pesquisas Gon莽alo Moniz ; FIOCRUZ ; Rua Waldemar Falc茫o ; 121 (Candeal) ; Salvador ; BA ; Brazil
  2. Laborat贸rio de Tecnologia Diagn贸stica ; Instituto de Tecnologia em Imunobiol贸gicos ; Bio-Manguinhos ; FIOCRUZ ; Rio de Janeiro ; RJ ; Brazil
  3. Instituto de Ci锚ncia e Tecnologia de Doen莽as Tropicais ; INCT-DT ; Salvador ; BA ; Brazil
  4. Laborat贸rio de Imunopatologia ; N煤cleo de Pesquisas em Ci锚ncias Biol贸gicas ; Universidade Federal de Ouro Preto ; Ouro Preto ; MG ; Brazil
  5. Departamento de Medicina Veterin谩ria Preventiva e Produ莽茫o Animal ; Escola de Medicina Veterin谩ria e Zootecnia ; Universidade Federal da Bahia ; Salvador ; BA ; Brazil
  
• 关键词：MAPIA ; Recombinant antigens ; Leishmaniasis
• 刊名：Parasites & Vectors
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：8
• 期：1
• 全文大小：623 KB
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• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background Domestic dogs are the principal reservoir hosts of Leishmania infantum in regions where visceral leishmaniasis (VL) is endemic. Although serologic methods are frequently used for the screening of infected dogs, antibody-based tests require further assessment, due to lack of sensitivity and specificity. In this study, we employed a multi-antigen printing immunoassay (MAPIA) to compare the antibody responses to novel recombinant proteins of L. infantum with the potential for the detection of canine VL. Findings MAPIA strips were prepared employing 12 recombinant proteins. Antibody reactivity to these antigens was compared using a panel of sera collected from clinically asymptomatic (n = 16) and symptomatic (n = 41) culture-positive animals. Our findings showed that the canine immune response to antigen differs between dogs and depends on infection status. Using this screening assay, when five out of the 12 antigens were combined, an overall 81% detection rate of L. infantum-infected dogs was achieved. Conclusions We conclude that MAPIA is an effective screening tool to rapidly select multiple antigens of diagnostic utility to be used in a more sensitive point of care diagnostic test such as the Dual-Path Platform (DPP) multiplex test for the rapid detection of infected dogs.