Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

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• 作者：Elaine Maria Seles Dorneles (86)
  Jordana Almeida Santana (86)
  Telma Maria Alves (86)
  Rebeca Barbosa Pauletti (86)
  Juliana Pinto da Silva Mol (86)
  Marcos Bryan Heinemann (86) (87)
  Andrey Pereira Lage (86)
  
  86. Laborat贸rio de Bacteriologia Aplicada ; Departamento de Medicina Veterin谩ria Preventiva ; Escola de Veterin谩ria ; Universidade Federal de Minas Gerais ; Av. Ant么nio Carlos ; 6627 ; Caixa Postal 567 ; 31270-901 ; Belo Horizonte ; MG ; Brazil
  87. Departamento de Medicina Veterin谩ria Preventiva e Sa煤de Animal - VPS ; Faculdade de Medicina Veterin谩ria e Zootecnia da Universidade de S茫o Paulo ; Av. Prof. Dr. Orlando Marques de Paiva ; 87 - Cidade Universit谩ria ; 05508-270 ; S茫o Paulo ; SP ; Brazil
  
• 关键词：Genotyping ; MLVA16 stability ; Bovine brucellosis ; B. abortus
• 刊名：BMC Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：373 KB
• 参考文献：1. Corbel, MJ, Elberg, SS, Cosivi, O (2006) Brucellosis in humans and animals. WHO Press, Geneva
  2. Poester, FP, Gon莽alves, VS, Lage, AP (2002) Brucellosis in Brazil. Vet Microbiol 90: pp. 55-62 CrossRef
  Minist茅rio da Agricultura, Pecu谩ria e Abastecimento. Instru莽茫o Normativa n潞 6, de 08 de janeiro de 2004. Aprova o Regulamento T茅cnico do Programa Nacional de Controle e Erradica莽茫o da Brucelose e da Tuberculose Animal 鈥?PNCEBT. Di谩rio Oficial da Uni茫o, Bras铆lia, DF
  3. Bricker, BJ (2002) PCR as a diagnostic tool for brucellosis. Vet Microbiol 90: pp. 435-446 CrossRef
  4. Struelens, MJ (1998) Molecular epidemiologic typing systems of bacterial pathogens: current issues and perpectives. Mem Inst Oswaldo Cruz 93: pp. 581-585 CrossRef
  5. Le Fl猫che, P, Jacques, I, Grayon, M, Al Dahouk, S, Bouchon, P, Denoeud, F, N枚ckler, K, Neubauer, H, Guilloteau, LA, Vergnaud, G (2006) Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 6: pp. 9 CrossRef
  6. Al Dahouk, S, Fl猫che, PL, N枚ckler, K, Jacques, I, Grayon, M, Scholz, HC, Tomaso, H, Vergnaud, G, Neubauer, H (2007) Evaluation of Brucella MLVA typing for human brucellosis. J Microbiol Methods 69: pp. 137-145 CrossRef
  7. Garc铆a-Yoldi, D, Le Fleche, P, Mar铆n, CM, De Miguel, MJ, Mu帽oz, PM, Vergnaud, G, L贸pez-Go帽i, I (2007) Assessment of genetic stablility of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable number tandem repeat analysis. Vaccine 25: pp. 2858-2862 CrossRef
  8. Her, M, Kang, SI, Cho, DH, Cho, YS, Hwang, IY, Heo, YR, Jung, SC, Yoo, HS (2009) Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea. BMC Microbiol 9: pp. 230 CrossRef
  9. 脕lvarez, J, S谩ez, JL, Garc铆a, N, Serrat, C, P茅rez-Sancho, M, Gonz谩lez, S, Ortega, MJ, Gou, J, Carbajo, L, Garrido, F, Goyache, J, Dom铆nguez, L (2011) Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain. Res Vet Sci 90: pp. 208-211 CrossRef
  10. Minharro, S, Mol, JP, Dorneles, EMS, Barbosa, RP, Neubauer, H, Melzer, F, Poester, FP, Dasso, MG, Pinheiro, ES, Soares Filho, PM, Santos, RL, Heinemann, MB, Lage, AP (2013) Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008. PLoS One 8: pp. 12 CrossRef
  11. Scholz, HC, Hubalek, Z, Sedl谩cek, I, Vergnaud, G, Tomaso, H, Al Dahouk, S, Melzer, F, K盲mpfer, P, Neubauer, H, Cloeckaert, A, Maquart, M, Zygmunt, MS, Whatmore, AM, Falsen, E, Bahn, P, G枚llner, C, Pfeffer, M, Huber, B, Busse, HJ, N枚ckler, K (2008) Brucella microti sp. nov., isolated from the commom vole Microtus arvalis. Int J Syst Evol Microbiol 58: pp. 375-382 CrossRef
  12. Marianelli, C, Petrucca, A, Pasquali, P, Ciuchini, F, Papadoulou, S, Cipriani, P (2008) Use of MLVA-16 typing to trace the source of a laboratory-acquired Brucella infection. J Hosp Infect 68: pp. 274-627 CrossRef
  13. Hunter, PR (1990) Reproducibility and indices of discriminatory power of microbial typing methods. J Clin Microbiol 28: pp. 1903-1905
  14. Whatmore, AM, Shankster, SJ, Perrett, LL, Murphy, TJ, Brew, SD, Thirlwall, RE, Cutler, SJ, MacMillan, AP (2006) Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J Clin Microbiol 44: pp. 1982-1993 CrossRef
  15. Dorneles, ESM, Faria, APP, Pauletti, RB, Santana, JA, Caldeira, GAV, Heinemann, MB, Titze-de-Almeida, R, Lage, AP (2013) Genetic stability of Brucella abortus S19 and RB51 vaccine strains by multiple locus variable number tandem repeat analysis (MLVA16). Vaccine 31: pp. 4856-4859 CrossRef
  16. Miranda, KL, Poester, FP, Minharro, S, Dorneles, EM, Stynen, AP, Lage, AP (2013) Evaluation of Brucella abortus S19 vaccines commercialized in Brazil: immunogenicity, residual virulence and MLVA15 genotyping. Vaccine 31: pp. 3014-3018 CrossRef
  17. Baily, GG, Krahn, JB, Drasar, BS, Stocker, NG (1992) Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95: pp. 271-275
  18. Bricker, B, Halling, S (1995) Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J Clin Microbiol 33: pp. 1640-1642
  19. L貌pez-Go帽i, I, Garc铆a-Yoldi, D, Mar铆n, CM, De Miguel, MJ, Mu帽oz, PM, Blasco, JM, Jacques, I, Grayon, M, Cloeckaert, A, Ferreira, AC, Cardoso, R, Corr锚a De S谩, MI, Walravens, K, Albert, D, Garin-Bastuji, B (2008) Evaluation of a multiplex PCR Assay (Bruce-Ladder) for molecular typing all Brucella species, including the vaccine strains. J Clin Microbiol 46: pp. 3484-3487 CrossRef
  20. Struelens, MJ (2002) Molecular typing: a key tool for the surveillance and control of nosocomial infection. Curr Opin Infect Dis 15: pp. 383-385 CrossRef
  21. Minharro, S (2009) Isolamento, Tipifica莽茫o e Genotipagem de Brucella abortus isoladas de bovinos no Brasil. PhD Thesis. Universidade Federal de Minas Gerais, Escola de Veterin谩ria
  22. Samartino, LE, Fort, M, Gregoret, R, Schurig, GG (2000) Use of Brucella abortus vaccine strain RB51 in pregnant cows after calfhood vaccination with strain 19 in Argentina. Prev Vet Med 45: pp. 193-199 CrossRef
  23. Miranda, KL (2009) Evaluation of Brucellosis Vaccines in Brazil. PhD Thesis, Universidade Federal de Minas Gerais, Escola de Veterin谩ria
  24. Weinberg, ED (1984) Pregnancy-associated depression of cell-mediated immunity. Rev Infect Dis 6: pp. 814-831 CrossRef
  25. Pappas, G, Panagopoulou, P, Christou, L, Akritidis, N (2006) Brucella as a biological weapon. Cell Mol Life Sci 63: pp. 2229-2236 CrossRef
  26. Xavier, MN, Paix茫o, TA, Poester, FP, Lage, AP, Santos, RL (2009) Pathological, immunohistochemical and bacteriological study of tissues and milk of cows and fetuses experimentally infected with Brucella abortus. J Comp Pathol 140: pp. 149-157 CrossRef
  27. Crawford, RP, Huber, JD, Adama, BS Epidemiology and Surveillance. In: Nielsen, K, Duncan, JR eds. (1990) Animal Brucellosis. CRC Press, Boca Raton, pp. 131-152
  28. Nielsen, K (2002) Diagnosis of brucellosis by serology. Vet Microbiol 90: pp. 447-459 CrossRef
  29. Nicoletti, P (1980) The epidemiology of bovine brucellosis. Adv Vet Sci Comp Med 24: pp. 69-98
  30. Alton, GG, Jones, LM, Angus, RD, Verger, JM (1988) Techniques for the brucellosis laboratory. INRA, Paris
  31. MacFaddin, JF (1980) Pruebas bioqu谋鈥檓icas para la identificacion de bact茅rias de import芒ncia clinica. Panamericana, Buenos Aires
  32. Miranda, KL, Dorneles, EMS, Poester, FP, Soares-Filho, PM, Barbosa, RP, Lage, AP (2013) Different resistance patterns of reference and field strains of Brucella abortus. Braz JMicrobiol.
  33. Pitcher, DG, Saunders, NA, Owern, RJ (1989) Rapid extraction of bacterial genomic DNA with guanidium thyocianate. Lett App Microbiol 8: pp. 151-156 CrossRef
  34. Sambrook, J, Russel, D (2001) Molecular cloning: a laboratory manual. CSHL Press, Plymouth
  
• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic diversity of B. abortus isolates from a brucellosis outbreak, and (ii) to investigate the in vivo stability of the MLVA16 markers. Results Three-hundred and seventy-five clinical samples, including 275 vaginal swabs and 100 milk samples, were cultured from a brucellosis outbreak in a cattle herd, which adopted RB51 vaccination and test-and-slaughter policies. Thirty-seven B. abortus isolates were obtained, eight from milk and twenty-nine from post-partum/abortion vaginal swabs, which were submitted to biotyping and genotyping by MLVA16. Twelve B. abortus isolates obtained from vaginal swabs were identified as RB51. Twenty four isolates, seven obtained from milk samples and seventeen from vaginal swabs, were identified as B. abortus biovar 3, while one isolate from vaginal swabs was identified as B. abortus biovar 1. Three distinct genotypes were observed during the brucellosis outbreak: RB observed in all isolates identified as RB51; W observed in all B. abortus biovar 3 isolates; and Z observed in the single B. abortus biovar 1 isolate. Epidemiological and molecular data show that the B. abortus biovar 1 genotype Z strain is not related to the B. abortus biovar 3 genotype W isolates, and represents a new introduction B. abortus during the outbreak. Conclusions The results of the present study on typing of multiple clinical B. abortus isolates from the same outbreak over a sixteen month period indicate the in vivo stability of MLVA16 markers, a low genetic diversity among B. abortus isolates and the usefulness of MLVA16 for epidemiological studies of bovine brucellosis.