miR-126 inhibits cell proliferation and induces cell apoptosis of hepatocellular carcinoma cells partially by targeting Sox2

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• 作者：Chunfang Zhao (1) (2)
  Ya Li (1) (2)
  Ming Zhang (1) (2)
  Yi Yang (1) (2)
  Li Chang (1) (2)
  
  1. Department of Radiation Oncology ; The Third Affiliated Hospital of Kunming Medical University ; Kunming ; 650118 ; Yunnan ; People鈥檚 Republic of China
  2. Department of Radiation Oncology ; The Tumor Hospital of Yunnan Province ; No. 519 of Kunzhou Road ; Kunming ; 650118 ; Yunnan ; People鈥檚 Republic of China
  
• 关键词：Hepatocellular carcinoma ; miR ; 126 ; Sox2 ; Cell proliferation ; Cell cycle ; Cell apoptosis
• 刊名：Human Cell
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：28
• 期：2
• 页码：91-99
• 全文大小：1,985 KB
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• 刊物主题：Cell Biology; Embryology; Oncology; Stem Cells; Reproductive Medicine; Cell Culture;
• 出版者：Springer Japan
• ISSN：1749-0774

文摘

Hepatocellular carcinoma (HCC) is the fifth most common malignancy and the third leading cause of cancer-related death globally. MicroRNAs (miRNAs) represent a new cohort of gene regulators. Currently, a large number of miRNAs have been reported to be associated with the initiation and maintenance of HCC. Through evaluating the relative concentrations of HCC-associated circulating miRNAs, underexpression of miR-126 has been identified in the blood of HCC patients. However, the exact function of miR-126 on HCC cellular biology progression and relative mechanisms were unclear. In this paper, we explored the function of miR-126 on HCC cells through exogenously transfecting HCC cells with miR-126 mimic. Restored miR-126 expression inhibited cell proliferation, arrest cell cycle progression, and induced cell apoptosis of HepG2 HCC cells. Moreover, to explore the mechanism of miR-126-mediated tumor suppression, we searched the putative targets of miR-126 using prediction program. Surprisingly, we found that sex-determining region Y-box 2 (Sox2) was a putative target gene of miR-126. Further luciferase assays, mRNA and protein assays consistently validated the target role of Sox2. Through restoring the expression of Sox2 in miR-126-transfected HepG2 cells, we found that overexpression of Sox2 could partially abrogate the miR-126-mediated suppression of cell growth. Thus, our data identified miR-126 as a tumor suppressor in HCC through, at least partially by targeting Sox2. This may provide novel diagnostic and therapeutic options for human HCC in future.