NADPH oxidase 1-dependent ROS is crucial for TLR4 signaling to promote tumor metastasis of non-small cell lung cancer

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• 作者：Xiyu Liu (1)
  Changyan Pei (2)
  Song Yan (3)
  Guifeng Liu (4)
  Gang Liu (3)
  Wenqing Chen (3)
  Youbin Cui (1)
  Yahui Liu (5)
  
  1. Department of Chest Surgery ; The First Bethune Hospital ; Jilin University ; 71 Xinminda Street ; Changchun City ; 130021 ; Jilin ; China
  2. Department of Medical Administration ; Jilin Province People鈥檚 Hospital ; Changchun ; 130000 ; Jilin ; China
  3. Department of Chest Surgery ; Jilin Province Cancer Hospital ; Changchun ; 130012 ; Jilin ; China
  4. Department of Radiology ; China-Japan Union Hospital of Jilin University ; Changchun ; 130000 ; Jilin ; China
  5. Department of Hepatobiliary and Pancreatic Surgery ; The First Bethune Hospital ; Jilin University ; 71 Xinminda Street ; Changchun City ; 130021 ; Jilin ; China
  
• 关键词：Non ; small cell lung cancer ; TLR4 ; NADPH oxidase 1 ; Tumor metastasis
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：36
• 期：3
• 页码：1493-1502
• 全文大小：418 KB
• 参考文献：1. Kanne, JP (2014) Screening for lung cancer: what have we learned?. AJR Am J Roentgenol 202: pp. 530-5 CrossRef
  2. Govindan, R, Bogart, J, Vokes, EE (2008) Locally advanced non-small cell lung cancer: the past, present, and future. J Thorac Oncol 3: pp. 917-28 CrossRef
  3. Siegel, R, Ward, E, Brawley, O, Jemal, A (2011) Cancer statistics, 2011: the impact of eliminating socioeconomic and racial disparities on premature cancer deaths. CA Cancer J Clin 61: pp. 212-36 CrossRef
  4. Li, Q, Han, Y, Fei, G, Guo, Z, Ren, T, Liu, Z (2012) IL-17 promoted metastasis of non-small-cell lung cancer cells. Immunol Lett 148: pp. 144-50 CrossRef
  5. Suleiman, AA, Nogova, L, Fuhr, U (2013) Modeling NSCLC progression: recent advances and opportunities available. AAPS J 15: pp. 542-50 CrossRef
  6. Dang, S, Peng, Y, Ye, L, Wang, Y, Qian, Z, Chen, Y (2013) Stimulation of TLR4 by LMW-HA induces metastasis in human papillary thyroid carcinoma through CXCR7. Clin Dev Immunol 2013: pp. 712561
  7. Zhang, D, Li, YH, Mi, M, Jiang, FL, Yue, ZG, Sun, Y (2013) Modified apple polysaccharides suppress the migration and invasion of colorectal cancer cells induced by lipopolysaccharide. Nutr Res 33: pp. 839-48 CrossRef
  8. Hsu, RY, Chan, CH, Spicer, JD, Rousseau, MC, Giannias, B, Rousseau, S (2011) LPS-induced TLR4 signaling in human colorectal cancer cells increases beta1 integrin-mediated cell adhesion and liver metastasis. Cancer Res 71: pp. 1989-98 CrossRef
  9. Liao, SJ, Zhou, YH, Yuan, Y, Li, D, Wu, FH, Wang, Q (2012) Triggering of Toll-like receptor 4 on metastatic breast cancer cells promotes 伪v尾3-mediated adhesion and invasive migration. Breast Cancer Res Treat 133: pp. 853-63 CrossRef
  10. Jing, YY, Han, ZP, Sun, K, Zhang, SS, Hou, J, Liu, Y (2012) Toll-like receptor 4 signaling promotes epithelial-mesenchymal transition in human hepatocellular carcinoma induced by lipopolysaccharide. BMC Med 10: pp. 98 CrossRef
  11. Zhan, Z, Xie, X, Cao, H, Zhou, X, Zhang, XD, Fan, H (2014) Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination. Autophagy 10: pp. 257-68 CrossRef
  12. Li, C, Li, H, Jiang, K, Li, J, Gai, X (2014) TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-尾1 and IL-10 and tumor cells migration. Biomed Mater Eng 24: pp. 869-75
  13. Ritsick, DR, Edens, WA, McCoy, JW, Lambeth, JD (2004) The use of model systems to study biological functions of Nox/Duox enzymes. Biochem Soc Symp 71: pp. 85-96
  14. Arbiser, JL, Petros, J, Klafter, R, Govindajaran, B, McLaughlin, ER, Brown, LF (2002) Reactive oxygen generated by Nox1 triggers the angiogenic switch. Proc Natl Acad Sci U S A 99: pp. 715-20 30199" target="_blank" title="It opens in new window">CrossRef
  15. Chamulitrat, W (2010) Role of gp91phox homolog nox1 in induction of premalignant spindle phenotypes of HPV 16 E6/E7-immortalized human keratinocytes. Sci World J 10: pp. 1435-49 CrossRef
  16. Ushio-Fukai, M, Nakamura, Y (2008) Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy. Cancer Lett 266: pp. 37-52 CrossRef
  17. Coso, S, Harrison, I, Harrison, CB, Vinh, A, Sobey, CG, Drummond, GR (2012) NADPH oxidases as regulators of tumor angiogenesis: current and emerging concepts. Antioxid Redox Signal 16: pp. 1229-47 CrossRef
  18. Xia, C, Meng, Q, Liu, LZ, Rojanasakul, Y, Wang, XR, Jiang, BH (2007) Reactive oxygen species regulate angiogenesis and tumor growth through vascular endothelial growth factor. Cancer Res 67: pp. 10823-30 CrossRef
  19. Wen, Z, Xu, L, Chen, X, Xu, W, Yin, Z, Gao, X (2013) Autoantibody induction by DNA-containing immune complexes requires HMGB1 with the TLR2/microRNA-155 pathway. J Immunol 190: pp. 5411-22 301" target="_blank" title="It opens in new window">CrossRef
  20. Yan, L, Cai, Q, Xu, Y (2013) The ubiquitin-CXCR4 axis plays an important role in acute lung infection-enhanced lung tumor metastasis. Clin Cancer Res 19: pp. 4706-16 CrossRef
  21. Fu, HY, Li, C, Yang, W, Gai, XD, Jia, T, Lei, YM (2013) FOXP3 and TLR4 protein expression are correlated in non-small cell lung cancer: implications for tumor progression and escape. Acta Histochem 115: pp. 151-7 CrossRef
  22. Zhang, YB, He, FL, Fang, M, Hua, TF, Hu, BD, Zhang, ZH (2009) Increased expression of Toll-like receptors 4 and 9 in human lung cancer. Mol Biol Rep 36: pp. 1475-81 CrossRef
  23. Hattar, K, Savai, R, Subtil, FS, Wilhelm, J, Schmall, A, Lang, DS (2013) Endotoxin induces proliferation of NSCLC in vitro and in vivo: role of COX-2 and EGFR activation. Cancer Immunol Immunother 62: pp. 309-20 CrossRef
  24. O鈥橪eary, DP, Bhatt, L, Woolley, JF, Gough, DR, Wang, JH, Cotter, TG (2012) TLR-4 signalling accelerates colon cancer cell adhesion via NF-魏B mediated transcriptional up-regulation of Nox-1. PLoS One 7: pp. e44176 CrossRef
  25. Maraldi, T (2013) Natural compounds as modulators of NADPH oxidases. Oxidative Med Cell Longev 2013: pp. 271602 CrossRef
  26. Bedard, K, Krause, KH (2007) The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 87: pp. 245-313 CrossRef
  27. Griffith, B, Pendyala, S, Hecker, L, Lee, PJ, Natarajan, V, Thannickal, VJ (2009) NOX enzymes and pulmonary disease. Antioxid Redox Signal 11: pp. 2505-16 CrossRef
  28. Cheng, SE, Lee, IT, Lin, CC, Wu, WL, Hsiao, LD, Yang, CM (2013) ATP mediates NADPH oxidase/ROS generation and COX-2/PGE2 expression in A549 cells: role of P2 receptor-dependent STAT3 activation. PLoS One 8: pp. e54125 CrossRef
  29. Cattaneo, F, Iaccio, A, Guerra, G, Montagnani, S, Ammendola, R (2011) NADPH-oxidase-dependent reactive oxygen species mediate EGFR transactivation by FPRL1 in WKYMVm-stimulated human lung cancer cells. Free Radic Biol Med 51: pp. 1126-36 CrossRef
  30. Kamata, T (2009) Roles of Nox1 and other Nox isoforms in cancer development. Cancer Sci 100: pp. 1382-8 CrossRef
  31. Laurent, E, McCoy, JW, Macina, RA, Liu, W, Cheng, G, Robine, S (2008) Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-Ras. Int J Cancer 123: pp. 100-7 CrossRef
  32. Komatsu, D, Kato, M, Nakayama, J, Miyagawa, S, Kamata, T (2008) NADPH oxidase 1 plays a critical mediating role in oncogenic Ras-induced vascular endothelial growth factor expression. Oncogene 27: pp. 4724-32 CrossRef
  33. Kim, JS, Yeo, S, Shin, DG, Bae, YS, Lee, JJ, Chin, BR (2010) Glycogen synthase kinase 3beta and beta-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages. FEBS J 277: pp. 2830-7 CrossRef
  34. Shinohara, M, Adachi, Y, Mitsushita, J, Kuwabara, M, Nagasawa, A, Harada, S (2010) Reactive oxygen generated by NADPH oxidase 1 (Nox1) contributes to cell invasion by regulating matrix metalloprotease-9 production and cell migration. J Biol Chem 285: pp. 4481-8 CrossRef
  35. Choi, YH, Burdick, MD, Strieter, BA, Mehrad, B, Strieter, RM (2014) CXCR4, but not CXCR7, discriminates metastatic behavior in non-small cell lung cancer cells. Mol Cancer Res 12: pp. 38-47 CrossRef
  36. Pei J, Lou Y, Zhong R, Han B. MMP9 activation triggered by epidermal growth factor induced FoxO1 nuclear exclusion in non-small cell lung cancer. Tumour Biol. 2014; 35:6673鈥?
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Recent evidence demonstrated an enhanced metastasis of non-small cell lung cancer (NSCLC) cells induced by lipopolysaccharide (LPS) stimulation, which reflected an important role of inflammation in tumor progression. However, the underlying mechanisms still remain unclear. Here, we evaluated the potential role of reactive oxygen species (ROS) in Toll-like receptor 4 (TLR4) signaling enhanced NSCLC metastasis. NSCLC cells were isolated from clinical surgical tissues. We found that LPS stimulation of NSCLC cells facilitates their metastasis that was accompanied by increased ROS production and could be abrogated by ROS inhibition. NADPH oxidase was essential for TLR4 signaling-enhanced NSCLC metastasis. Elevated NADPH oxidase 1 (NOX1) expression by LPS stimulation was observed. Blockade of NOX1 with ML171 alleviated enhanced NSCLC metastasis by TLR4 signaling. Enforced NOX1 expression promoted TLR4 signaling-enhanced NSCLC metastasis, while decreased NOX1 expression inhibited TLR4 signaling-enhanced NSCLC metastasis. Further, NOX1 could regulate the expression of CXCR4 and matrix metallopeptidase 9 (MMP9) in NSCLC cells. NOX1 expression in tumor tissues was correlated with TLR4 expression and clinical stages in NSCLC patients. Finally, inhibition of NOX1/ROS prevented enhanced lung tumor burdens of NSCLC by LPS-induced acute lung infection. Our findings demonstrated a crucial role of NOX1-dependent ROS for TLR4 signaling to enhance the metastasis of NSCLC, which could further the understanding of NSCLC pathogenesis and helpful for developing novel therapeutics for NSCLC.