Identification of flavonoids from Eriodictyon californicum and their cytotoxicity against HCT116 colon cancer cells

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• 作者：Soon Young Shin (1)
  Yeonjoong Yong (2)
  Dae Sun Hong (3)
  Da Hyun Lee (1)
  Da Young Lee (4)
  Young Han Lee (1)
  
  1. Department of Biological Sciences ; Cancer and Metabolism Institute ; College of Biological Science and Biotechnology ; Konkuk University ; Seoul ; 143-701 ; Korea
  2. Division of Bioscience and Biotechnology ; BMIC ; Konkuk University ; Seoul ; 143-701 ; Korea
  3. California Acupuncture Herbal Medicine and Homeopathy ; Fullerton ; CA ; 92831 ; USA
  4. Division of Biological Sciences ; Biology w/Spec Bioinformatics ; Revelle College ; University of California ; San Diego ; CA ; 92093 ; USA
  
• 关键词：Eriodictyon californicum ; Antitumor activity ; Flavonoid ; Caspase ; Apoptosis ; Colon cancer
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：58
• 期：1
• 页码：77-81
• 全文大小：785 KB
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• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

The extract of Eriodictyon californicum has been widely used as an essential emollient, and as an anti-inflammatory, antibacterial, and antifungal agent. In this study, antitumor effects of E. californicum were evaluated against HCT116 human colon cancer cells. Treatment with the ethyl acetate extract of E. californicum (EEC) inhibited the short-term viability and long-term clonogenicity of HCT116 cells. We identified several flavonoids, including rosmarinic acid and luteolin, from the EEC by using high-performance liquid chromatography and nuclear magnetic resonance. Luteolin inhibited clonogenicity, activated caspase-7, and induced apoptosis of HCT116 cells. These findings suggest that EEC contains bioactive flavonoids that exhibit antitumor activities.