MicroRNA-21 controls hTERT via PTEN in human colorectal cancer cell proliferation

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• 作者：Yang Yang (1) (2)
  Jing-Jing Yang (3)
  Hui Tao (4)
  Wei-Sen Jin (1) (2)
  
  1. Clinical College of General Hospital of Armed Police Forces ; Anhui Medical University ; Hefei ; 230032 ; China
  2. Department of Anorectal ; General Hospital of Armed Police Forces ; Beijing ; 100039 ; China
  3. Department of Pharmacology ; The Second Hospital of Anhui Medical University ; Hefei ; 230601 ; China
  4. Department of Cardiothoracic Surgery ; The Second Hospital of Anhui Medical University ; Hefei ; 230601 ; China
  
• 关键词：MicroRNA ; 21 ; Colorectal cancer ; Human telomerase reverse transcriptase ; Phosphatase and tensin homologue
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：71
• 期：1
• 页码：59-68
• 全文大小：1,045 KB
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• 刊物主题：Biomedicine general; Human Physiology; Animal Physiology;
• 出版者：Springer Netherlands
• ISSN：1877-8755

文摘

Colorectal cancer is a major health problem worldwide. The aim of this study was to determine a role of microRNA-21 (miRNA-21) in colorectal cancer (CRC) and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue (PTEN). Protein and mRNA samples were extracted with 30 CRC samples and one CRC cell lines. The expression of miRNA-21, hTERT, PTEN in CRC tissues and CRC cell lines was measured by real-time fluorescent quantitative PCR, Western blotting. Cell viability was detected by MTT and cell cycle assay. In this study, we show that the expression of miRNA-21 was overexpressed in CRC tissue and CRC cell lines compared with the control group. The effects of miRNA-21 were then assessed in MTT assays through in vitro transfection with a miRNA-21 mimic or inhibitor. PTEN has been identified as a target gene of miRNA-21 in CRC cell lines. Moreover, Western blot and qRT-PCR analyses revealed that miRNA-21 increased the expression of human telomerase reverse transcriptase (hTERT) via the PTEN/ERK1/2. In addition, Western blot analyses confirmed that an inverse correlation between PTEN and hTERT levels of high miRNA-21 RNA-expressing CRC tissues and cell lines. Finally, these data indicate that miRNA-21 regulates hTERT expression via the PTEN/ERK1/2 signaling pathway, therefore controlling CRC cell line growth. MiRNA-21 may serve as a novel therapeutic target in CRC.