Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer

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• 作者：Zhujiang Zhao (1)
  Ling Wang (1)
  Wei Song (1)
  He Cui (1)
  Gang Chen (2)
  Fengchang Qiao (1)
  Jiaojiao Hu (1)
  Rongping Zhou (2)
  Hong Fan (1)
  
  1. Department of Genetics & Developmental Biology ; the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases ; Ministry of Education ; Southeast University ; 2 Sipailou ; Xuanwu ; Nanjing ; 210009 ; China
  2. Jiangning Hospital ; Nanjing Medical University ; 140 Hanzhong Road ; Nanjing ; 210000 ; China
  
• 关键词：miR ; 29a ; 3p ; Migration ; Cell proliferation ; Gastric cancer
• 刊名：World Journal of Surgical Oncology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：13
• 期：1
• 全文大小：2,347 KB
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• 刊物主题：Surgical Oncology;
• 出版者：BioMed Central
• ISSN：1477-7819

文摘

Background MicroRNAs (miRNAs) play an important role in a tumor-suppressive or oncogenic manner in carcinogenesis. Alteration expression patterns of miRNAs in gastric cancer (GC) are associated with cancer initiation and progression. In the present study, we evaluated miR-29a-3p expression pattern and its function in gastric carcinogenesis. Methods The expression of miR-29a-3p in GC tissue samples and cell lines was detected by quantitative real-time PCR (qRT-PCR). After transfected with miR-29a-3p mimics or inhibitor, the cell proliferation, cell migration, and invasion ability were assessed by CCK-8 assay, wound healing assay, and Trans-well assay, respectively. The level of CDK2, CDK4, CDK6, and CyclinD1 were determined by qRT-PCR and Western blot. Results Compared with the corresponding non-tumor tissues, miR-29a-3p showed a significant down-regulated expression in tumor tissues. In vitro functional assays demonstrated that enforced miR-29a-3p expression inhibited cell proliferation by reducing the expression of CDK2, CDK4, and CDK6. Wound healing and Transwell assays revealed that miR-29a-3p suppressed tumor metastasis in GC. Conclusions Our preliminary results suggest that altered expression of miR-29a-3p is involved in gastric cancer process. The present study provides the first insight into the specific role of miR-29a-3p in gastric carcinogenesis.