Alteration of Gene Expression Profiling Including GPR174 and GNG2 is Associated with Vasovagal Syncope

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• 作者：Yu-Juan Huang (1) (2) (3) (4)
  Zai-wei Zhou (1) (4)
  Miao Xu (1) (4)
  Qing-wen Ma (1) (4)
  Jing-bin Yan (1) (4)
  Jian-yi Wang (2)
  Quo-qin Zhang (3)
  Min Huang (2)
  Liming Bao (1) (5)
  
  1. Shanghai Institute of Medical Genetics ; Shanghai Children鈥檚 Hospital ; Shanghai Jiao Tong University School of Medicine ; Shanghai ; China
  2. Department of Cardiology ; Shanghai Children鈥檚 Hospital ; Shanghai Jiao Tong University School of Medicine ; Shanghai ; China
  3. Department of Emergency Medicine ; Shanghai Children鈥檚 Hospital ; Shanghai Jiao Tong University School of Medicine ; Shanghai ; China
  4. Key Lab of Embryo Molecular Biology & Shanghai Lab of Reproduction and Embryo Engineering ; Shanghai ; 200040 ; China
  5. Department of Pathology ; Geisel School of Medicine Dartmouth College ; Lebanon ; NH ; USA
  
• 关键词：Vasovagal syncope ; G ; proteins ; Gene expression profile ; Pediatrics
• 刊名：Pediatric Cardiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：36
• 期：3
• 页码：475-480
• 全文大小：469 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Cardiology
  Cardiac Surgery
  Vascular Surgery
  
• 出版者：Springer New York
• ISSN：1432-1971

文摘

Vasovagal syncope (VVS) causes accidental harm for susceptible patients. However, pathophysiology of this disorder remains largely unknown. In an effort to understanding of molecular mechanism for VVS, genome-wide gene expression profiling analyses were performed on VVS patients at syncope state. A total of 66 Type 1 VVS child patients and the same number healthy controls were enrolled in this study. Peripheral blood RNAs were isolated from all subjects, of which 10 RNA samples were randomly selected from each groups for gene expression profile analysis using Gene ST 1.0 arrays (Affymetrix). The results revealed that 103 genes were differently expressed between the patients and controls. Significantly, two G-proteins related genes, GPR174 and GNG2 that have not been related to VVS were among the differently expressed genes. The microarray results were confirmed by qRT-PCR in all the tested individuals. Ingenuity pathway analysis and gene ontology annotation study showed that the differently expressed genes are associated with stress response and apoptosis, suggesting that the alteration of some gene expression including G-proteins related genes is associated with VVS. This study provides new insight into the molecular mechanism of VVS and would be helpful to further identify new molecular biomarkers for the disease.