USP7 overexpression predicts a poor prognosis in lung squamous cell carcinoma and large cell carcinoma

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• 作者：Guang-Yin Zhao (1)
  Zong-Wu Lin (1)
  Chun-Lai Lu (1)
  Jie Gu (1)
  Yun-Feng Yuan (1)
  Feng-Kai Xu (1)
  Rong-Hua Liu (2)
  Di Ge (1)
  Jian-Yong Ding (1)
  
  1. Department of Thoracic Surgery ; The Affiliated Zhongshan Hospital of Fudan University ; 200032 ; Shanghai ; People鈥檚 Republic of China
  2. Department of Immunology ; Shanghai Medical College ; Key Laboratory of Molecular Medicine of Ministry of Education ; Fudan University ; 200032 ; Shanghai ; People鈥檚 Republic of China
  
• 关键词：Lung cancer ; USP7 ; Prognosis ; Apoptosis
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：36
• 期：3
• 页码：1721-1729
• 全文大小：1,551 KB
• 参考文献：1. Jemal, A, Siegel, R, Xu, J, Ward, E (2010) Cancer statistics, 2010. CA Cancer J Clin 60: pp. 277-300 CrossRef
  2. Ferlay, J, Shin, HR, Bray, F, Forman, D, Mathers, C, Parkin, DM (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127: pp. 2893-917 CrossRef
  3. Li, M, Chen, D, Shiloh, A, Luo, J, Nikolaev, AY, Qin, J (2002) Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization. Nature 416: pp. 648-53 CrossRef
  4. Nicholson, B, Suresh, KK (2011) The multifaceted roles of USP7: new therapeutic opportunities. Cell Biochem Biophys 60: pp. 61-8 CrossRef
  5. Song, MS, Salmena, L, Carracedo, A, Egia, A, Lo-Coco, F, Teruya-Feldstein, J (2008) The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network. Nature 455: pp. 813-7 CrossRef
  6. Chauhan, D, Tian, Z, Nicholson, B, Kumar, KG, Zhou, B, Carrasco, R (2012) A small molecule inhibitor of ubiquitin-specific protease-7 induces apoptosis in multiple myeloma cells and overcomes bortezomib resistance. Cancer Cell 22: pp. 345-58 CrossRef
  7. Kon, N, Kobayashi, Y, Li, M, Brooks, CL, Ludwig, T, Gu, W (2010) Inactivation of HAUSP in vivo modulates p53 function. Oncogene 29: pp. 1270-9 CrossRef
  8. Masuya, D, Huang, C, Liu, D, Nakashima, T, Yokomise, H, Ueno, M (2006) The HAUSP gene plays an important role in non-small cell lung carcinogenesis through p53-dependent pathways. J Pathol 208: pp. 724-32 CrossRef
  9. Li, D, Lei, Y, Deng, J, Zhou, C, Zhang, Y, Li, W (2013) Human but not laboratory borna disease virus inhibits proliferation and induces apoptosis in human oligodendrocytes in vitro. PLoS ONE 8: pp. e66623 CrossRef
  10. Gu, J, Ding, JY, Lu, CL, Lin, ZW, Chu, YW, Zhao, GY (2013) Overexpression of CD88 predicts poor prognosis in non-small-cell lung cancer. Lung Cancer-J Iaslc 81: pp. 259-65 CrossRef
  11. Nicholson, B, Marblestone, JG, Butt, TR, Mattern, MR (2007) Deubiquitinating enzymes as novel anticancer targets. Future Oncol 3: pp. 191-9 CrossRef
  12. Amaral, JD, Xavier, JM, Steer, CJ, Rodrigues, CM (2010) The role of p53 in apoptosis. Discov Med 9: pp. 145-52
  13. Brooks, CL, Li, M, Hu, M, Shi, Y, Gu, W (2007) The p53鈥揗dm2鈥揌AUSP complex is involved in p53 stabilization by HAUSP. Oncogene 26: pp. 7262-6 CrossRef
  14. Cummins, JM, Rago, C, Kohli, M, Kinzler, KW, Lengauer, C, Vogelstein, B (2004) Tumour suppression: disruption of HAUSP gene stabilizes p53. Nature 428: pp. 1-486 CrossRef
  15. Li, M, Brooks, CL, Kon, N, Gu, W (2004) A dynamic role of HAUSP in the p53-Mdm2 pathway. Mol Cell 13: pp. 879-86 CrossRef
  16. Meulmeester, E, Maurice, MM, Boutell, C, Teunisse, AF, Ovaa, H, Abraham, TE (2005) Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of Hdmx and Hdm2. Mol Cell 18: pp. 565-76 CrossRef
  17. Bonni, A, Brunet, A, West, AE, Datta, SR, Takasu, MA, Greenberg, ME (1999) Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286: pp. 1358-62 CrossRef
  18. She, QB, Ma, WY, Zhong, S, Dong, Z (2002) Activation of JNK1, RSK2, and MSK1 is involved in serine 112 phosphorylation of Bad by ultraviolet B radiation. J Biol Chem 277: pp. 24039-48 CrossRef
  19. Everett, RD, Meredith, M, Orr, A, Cross, A, Kathoria, M, Parkinson, J (1997) A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein. EMBO J 16: pp. 1519-30 CrossRef
  20. Hu, M, Li, P, Li, M, Li, W, Yao, T, Wu, JW (2002) Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde. Cell 111: pp. 1041-54 CrossRef
  21. Jones, SN, Roe, AE, Donehower, LA, Bradley, A (1995) Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53. Nature 378: pp. 206-8 CrossRef
  22. Sheng, Y, Saridakis, V, Sarkari, F, Duan, S, Wu, T, Arrowsmith, CH (2006) Molecular recognition of p53 and MDM2 by USP7/HAUSP. Nat Struct Mol Biol 13: pp. 285-91 CrossRef
  23. Fridman, JS, Lowe, SW (2003) Control of apoptosis by p53. Oncogene 22: pp. 9030-40 CrossRef
  24. Gryko, M, Pryczynicz, A, Guzinska-Ustymowicz, K, Kamocki, Z, Zareba, K, Kemona, A (2012) Immunohistochemical assessment of apoptosis-associated proteins: P53, Bcl-xL, Bax and Bak in gastric cancer cells in correlation with clinical and pathomorphological factors. Adv Med Sci 57: pp. 77-83 CrossRef
  25. Yang, E, Zha, J, Jockel, J, Boise, LH, Thompson, CB, Korsmeyer, SJ (1995) Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 80: pp. 285-91 CrossRef
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

In non-small cell lung cancer (NSCLC), both USP7 expression and p53 gene status were reported to be an indicator of poor prognosis in adenocarcinoma patients; however, its roles and mechanisms in lung squamous cell carcinoma and large cell carcinoma need to be clarified. The USP7 expression was examined in NSCLC tumors (excluding adenocarcinoma), their corresponding non-tumorous tissues, and NSCLC cells. Then, the prognostic role of USP7 was analyzed in 110 NSCLC samples (excluding the adenocarcinoma). Finally, the roles and mechanisms of USP7 in the proliferation, metastasis, and invasion of a NSCLC cell were assessed using a specific vshRNA. The USP7 expression was higher in NSCLC tissues compared to non-tumorous samples, accordingly, the high level of USP7 was detected in NSCLC cell lines compared with HBE cell. After the USP7 downregulation, the H460 cells exhibited decreased metastasis/invasion in vitro and in vivo. The preliminary mechanism study indicated overexpression of USP7 might regulate the p53-MDM2 pathway by inducing the MDM2 de-ubiquitination and subsequent stabilization, which resulted in the upregulation of the Bad phosphorylation. Additionally, we also found that USP7 might induce cell epithelial-mesenchymal transition to enhance the cell invasive ability. Clinically, USP7 overexpression significantly correlated with malignant phenotype. Furthermore, the 5-year overall survival in patients with USP7low was higher than that of USP7high. Multivariate analysis showed USP7 overexpression was an independent prognostic marker for these cancers. USP7 overexpression may regulate the survival and invasive properties of squamous cell carcinoma and large cell carcinoma cells, and may serve as a molecular target.