Target-responsive DNAzyme hydrogel for portable colorimetric detection of lanthanide(III) ions

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• 作者：Yishun Huang ; Xuemeng Wu ; Tian Tian ; Zhi Zhu ; Hui Lin…
• 关键词：lanthanides ; DNAzyme ; colorimetric detection ; hydrogel
• 刊名：Science China Chemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：60
• 期：2
• 页码：293-298
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry/Food Science, general;
• 出版者：Science China Press
• ISSN：1869-1870
• 卷排序：60

文摘

Lanthanide elements (Ln) play an important role in industry and agriculture. As a result of the increasing consumption of lanthanides, environmental emission of Ln has become detrimental to the health of flora and fauna. Current methods for trace lanthanides detection mainly rely on sophisticated instruments. In this article, a Ln3+ dependent DNAzyme was incorporated into a hydrogel to generate Ln3+ sensitive DNAzyme hydrogel for portable colorimetric detection. The enzyme strand and its substrate strand act as crosslinker and functional unit of the hydrogel with polyacrylamide chains as the scaffold and gold nanoparticles (AuNPs) as the indicator of hydrogel stability. Any ions in the Ln3+ series can trigger the cleavage of substrate strand by activating the enzyme strand, thereby decreasing the crosslink ratio and leading to collapse of the hydrogel. The release of the encapsulated AuNPs turns the supernatant wine red. Using this colorimetric method, Ln3+ can be detected with high sensitivity, with a limit of detection (LOD) of 20 nM for Ce3+. The hydrogel responds specifically to any Ln3+ ion and works well with the spiked lake sample without the need of instruments and skilled operators. Our results suggest that the lanthanide responsive hydrogel can be used for portable and sensitive detection of Ln3+ contamination in the field.